鞘内注射右美托咪啶对小鼠急性炎性痛的影响  

Effect of intrathecal dexmedetomidine on acute inflammatory pain in mice

作　　者：张红星[1] 曹学照[2] 周芳[1] 孔敏[3] 孔德强[4] 孙学军[1] 荆娜[1] 马虹[2] 

机构地区：[1]中国医科大学第一临床学院,沈阳市110003 [2]中国医科大学第一附属医院麻醉科 [3]徐州医学院麻醉学重点实验室 [4]徐州医学院附属医院麻醉科

出　　处：《中华麻醉学杂志》2011年第12期1458-1460,共3页Chinese Journal of Anesthesiology

摘　　要：目的探讨鞘内注射右美托咪啶对小鼠急性炎性痛的影响。方法雄性成年昆明小鼠110只，体重18～20g，采用随机数字表法，将其随机分为5组（n=22）：二甲亚砜组（DMSO组）、炎性痛组（IP组）和不同剂量右美托咪啶组（D1-3组）。D1-3组分别鞘内注射右美托咪啶0．100、0．020和0．004μg／μl（溶于10μl人工脑脊液中）。于鞘内注射后5min时，IP组和D1-3组左后爪底皮内注射辣椒素0．1μg／μl（溶于10μl二甲基亚砜中），制备小鼠急性炎性痛模型；DMSO组爪底注射二甲基亚砜10μl。每组随机取8只小鼠，于爪底给药前1h（T0）、给药后即刻（T1）、5min（T2）和10min（T3）时测定热缩爪潜伏期；每组随机取8只小鼠，记录5min内出现舔／咬给药侧爪的时间。于爪底给药后5min时，每组随机处死6只小鼠，取脊髓L4-5段，采用Western blot法测定磷酸化ERK1／2（p-ERK1／2）和ERK1／2的表达。结果与DMSO组比较，IP组和D3组T1和T2时热缩爪潜伏期缩短，舔／咬爪时间延长，脊髓p-ERKl／2表达上调（P〈0．01），D1组和D1组T1-3，时热缩爪潜伏期延长（P〈0．01），舔／咬爪时间和脊髓p-ERK1／2表达差异无统计学意义（P〉0．05）；与IP组和D3组比较，D2组和D2组D2组T1-3，时热缩爪潜伏期延长，舔／咬爪时间缩短，脊髓p-ERK1／2表达下调（P〈0．05）；IP组和D，组间、D3组和D1组间热缩爪潜伏期、舔／咬爪时间和脊髓p-ERK1／2表达比较差异无统计学意义，各组间脊髓ERK1／2表达比较差异无统计学意义（P〉0．05）。结论鞘内注射右美托咪啶可减轻小鼠急性炎性痛，其机制与抑制脊髓ERK1／2的活性有关。Objective To investigate the effect of intrathecal dexmedetomidine on acute inflammatory pain in mice. Methods One hundred and ten aduh male Kunming mice, weighing 18-20 g, were randomly divided into 5 groups（ n = 22 each） ： dimethyl sulfoxide group（group DMSO） ; inflammatory pain group（group IP） and different doses of dexmedetomdine group （group D1-3 ）. Intrathecal dexmedetomdine 0. 100, 0.020 and 0.004 μg/μl （in 10 fd artificial cerebrospinal fluid） were injected in groups Dl 3 respectively. Five minutes later, inflammatory pain was induced by capsaicin 0.1 μg/μl （in 10 kd DMSO） given intracutaneously in the left hindpaw in groups IP and D1-3. While DMSO 10 μl was given intracutaneously in the left himlpaw in group DMSO. Thermal withdrawal latency was determined at 1 h before （TO ）, immediately, at 5 and 10 rain after intracutaneous injection （T,_3） in 8 mice in each group. Licking/biting time was recorded within 5 rain after intraeutaneous injection in 8 mice in each group. Six mice were sacrificed and L4_5 spinal cord was removed for determination of phosphorylated extracellular signal-regulated protein kinase 1/2 （p-ERK1/2） and ERK1/2 at 5 min after intracutaneous injection. Results Compared with group DMSO, the paw withdrawal latency was significantly shortened at T1 and T2, the licking/biting paw time prolonged and the expression of p-ERK1/2 up-regulated in groups IP and D3 , the paw withdrawal latency was significantly prolonged at T1-3 in groups D1 and D2 （P 〈 0.01 ） . Compared with groups IP and D3 , the paw withdrwal latency was significantly prolonged at T1-3, the licking/biting paw time shortened and the expression of p-ERK1/2 down-regulated in groups Dl and D2 （ P 〈 0.01 ）. There was no significant difference in the paw with-drawal latency, licking/biting paw time and expression of p-ERKI/2 between groups IP and D3 and between groups D1 and D2 （ P 〉 0.05）. There was no significant difference in the expression Of ERK1/2 among all the groups

关 键 词：右美托咪啶 炎症 疼痛 注射 脊髓 

分 类 号：R614[医药卫生—麻醉学]