鸡新城疫病毒新疆分离株F基因遗传进化分析  

Genetic Evolution Analysis on F Gene of Newcastle Disease Virus Xinjiang Isolates

作　　者：沙依兰古丽[1] 汪萍[1] 巴特力 陆桂丽[1] 成进[1] 

机构地区：[1]新疆畜牧科学院兽医研究所,新疆乌鲁木齐830000 [2]新疆维吾尔自治区动物卫生监督所,新疆乌鲁木齐830063

出　　处：《动物医学进展》2011年第12期25-30,共6页Progress In Veterinary Medicine

基　　金：2009年度新疆少数民族特殊培养项目(200923108)

摘　　要：对2株分离自新疆乌鲁木齐市的鸡源新城疫病毒F基因进行了扩增、序列测定和分析。结果显示,该2株新城疫病毒F基因核苷酸序列长度分别为1 586bp和1 654bp,分別编码520和545个氨基酸;氨基酸裂解位点的序列均为112R-R-Q-K-R-F117,具备了基因Ⅶ型强毒株的特征;同源性分析表明,该2株新城疫病毒分离株与现用疫苗毒株的同源性在81.8%～84.2%之间,与国内外流行的基因Ⅶ型新城疫病毒同源性在87.4%～98.2%之间,与基因Ⅶd亚型新城疫病毒株的同源性较高,为94.4%～98.2%;遗传进化分析表明,该2个毒株与FJ882014-LN、FJ608335-XJ、FJ80805-JL等国内流行的基因Ⅶd亚型新城疫病毒株在一个分支内亲缘关系最近。The F genes of two Newcastle disease virus （NDV） isolates, isolated from poultry farm in Urumqi were amplified and analyzed. The results showed that the nucleotide sequence lengths of F genes were 1 589 bp and 1 654 bp,respeetively,encoding 528 and 545 amino acids. The sequence of amino acid at the cleavage site of F gene is 112R-R-Q-K-R-F117, which consistent with the characteristics of Newcastle disease virus of gene Ⅶ. Comparison of the two NDV strains with the current vaccine strains showed that the nucleotide homology was 81.8%-84.2%. Comparison of two NDV strains with the NDV strains of gene Ⅶ of epidemic strains from different countries showed that the nucleotide homology was 87.4%-98.2 %, and compared the nucleotide homology was 94.4 %-98.2 % compared. The phylogenetic tree analysis demonstrated that the two NDV isolates and strains of FJ882014-LN, FJ608335-XJ and FJS0805-JL were located in the same cladogram branch. These strains all belong to NDV of the gene Ⅶd.

关 键 词：新城疫病毒强毒 F基因 序列分析 新疆株 鸡 

分 类 号：S852.659.5[农业科学—基础兽医学]