巴戟天对大鼠心肌缺血再灌注损伤的影响  

Effect of Radix morindae offieinalis on myoeardial ischemia-reperfusion injury in rats

作　　者：李凯[1] 司忠义[1] 李岩欣[1] 黄坤[1] 

机构地区：[1]辽宁医学院附属第一医院心脏大血管外科,辽宁锦州121001

出　　处：《军医进修学院学报》2012年第3期277-279,301,共4页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的观察巴戟天(radix morindae officinalis,RMO)对大鼠心肌缺血再灌注损伤的影响并探讨其可能机制。方法雄性大鼠40只,完全随机等分为假手术(sham operation,sham)组,心肌缺血/再灌注(myocardial ischemia/reperfusion,I/R)模型组,RMO分为高剂量组3.0g/(kg.d)、低剂量组1.2g/(kg.d),每组10只灌胃。Sham组和模型组给予等量蒸馏水灌胃,Sham组穿线不结扎,其余三组均穿线结扎冠状动脉左前降支(LAD)起始部30min,再灌注60min。观察心肌梗死面积、心肌组织中CK、NO、iNOS含量与Ca2+-Mg2+-ATPase、Na+-K+-ATPase活性的变化。结果与模型组相比,RMO各剂量组均可明显缩小心肌梗死面积(P<0.05),且心肌组织中Na+-K+-ATPase、Ca2+-Mg2+-ATPase、NO、iNOS活性升高,而CK含量降低(P<0.05)。结论 RMO有预防MIRI作用,其机制主要与防止钙超载及氧自由基有关。Objective To study the possible mechanism of radix morindae offieinalis（RMO） underlying myocardial ischemia-reperfusion injury（IRI） in rats by observing its effect on IRI.Methods Forty male rats were randomly divided into sham operation group,myocardial IRI model group,high-and low-dose RMO groups（10 rats in each group）.Rats in sham operation group and high-and low-dose RMO groups received a same volume of distilled water.Coronary artery in rats of sham operation group was not ligated,while the left anterior descending（LAD） of coronary artery in rats of the other 3 groups was ligated for 30min and reperfused for 60min.Then,their cardiac Infarction size,and creatine kinase（CK）,Na＋-K＋-ATPase,Ca2＋-Mg2＋-ATPase,nitric oxide（NO） and iNOS levels in myocardial tissue were measured.Results The cardiac infarction size was significantly smaller while the Na＋-K＋-ATPase,Ca2＋-Mg2＋-ATPase,NO and iNOS levels in myocardial tissue were higher and the CK level was lower in IRI model group than in high-and low-dose RMO groups（P0.05）.Conclusion RMO can protect myocardium against IRI in rats by preventing calcium overload and reducing oxygen free radicals.

关 键 词：巴戟天 缺血/再灌注 心肌预适应 ATP酶 一氧化氮 

分 类 号：R445.1[医药卫生—影像医学与核医学]