普通PCR与TD-PCR扩增叶尔羌高原鳅抗菌肽Hepcidin基因的比较  

Comparison of Ordinary PCR and Landing PCR Amplification ofHepcidin gene from Triplophysa(Hedinichthys) yarkandensis(Day)

作　　者：刘书东[1] 王娟娟 陈根元[2] 李莲瑞[2] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]塔里木畜牧科技兵团重点实验室,新疆阿拉尔843300

出　　处：《塔里木大学学报》2011年第4期40-45,共6页Journal of Tarim University

基　　金：新疆生产建设兵团博士资金(2009JCl8)

摘　　要：为了比较普通PCR和降落PCR(TD-PCR)扩增Hepcidin基因的效果,通过Trizol法提取叶尔羌高原鳅肝胰脏总RNA并合成cDNA,分别利用普通PCR和降落PCR扩增Hepcidin基因片段。结果显示,降落PCR能有效扩增叶尔羌高原鳅Hepci-din。测序结果显示:叶尔羌高原鳅抗菌肽Hepcidin基因与普通鱼类基因同源性达90%,而普通PCR则不能扩出明显目的条带。本研究结果表明在相同反应体系中,降落PCR在不影响PCR特异性的条件下,能提高扩增效率,可用于扩增普通PCR难以扩增的基因片段。In order to compare the effect of Hepcidin gene amplification by odinary PCR and the landing PCR（TD-PCR）,the cDNA from total RNA synthesis of Triplophysa yarkandensis＇s hepatopancreas is respectively used to amplify Hepcidin gene fragment using common PCR and landing PCR.The results showed that the landing PCRcan effectively amplified Hepcidin gene.The sequencing results showed that the Hepcidin gene homology of Triplophysa yarkandensis and the ordinary fish was up to 90%,while the ordinary PCR did not enlarge apparent purpose strip.The results of this study show that in the same reaction system,landing PCR without affecting the specificity,can increase amplification efficiency,and can be used for PCR amplification of ordinary hard-amplified gene fragments.

关 键 词：降落PCR 叶尔羌高原鳅 HEPCIDIN基因 

分 类 号：Q785[生物学—分子生物学]