巴西橡胶树抗坏血酸过氧化酶基因HbAPX的克隆及其对水杨酸的应答  

Cloning of Ascorbate Peroxidase Gene HbAPX in Hevea brasiliensis and Its Response to Salicylic Acid

作　　者：罗红丽[1] 闫志烨[1] 

机构地区：[1]海南大学海南省热带作物资源可持续利用重点实验室,海南海口570228

出　　处：《热带生物学报》2011年第3期197-203,共7页Journal of Tropical Biology

基　　金：海南大学"211工程"热带作物遗传育种与生态保育创新人才培养基金青年教师项目(QNJS-2011-08);海南大学"211工程"专项资金(研究生教育教学改革研究项目)资助;海南省自然科学基金项目(311030)

摘　　要：在对橡胶树转录组进行测序的基础上,利用模式植物中抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)基因的氨基酸序列进行比对并设计引物,通过RT-PCR方法扩增巴西橡胶树APX基因的cDNA片段,命名为HbAPX。该片段包含1个858核苷酸的开放阅读框,编码285个氨基酸的多肽。生物信息学分析结果表明,该蛋白氨基酸序列与麻风树(Jatropha curcas)的APX高度同源,同源性达90%,与杨树(Populus tomen-tosa)、陆地棉(Gossypium hirsutum)、拟南芥(Arabidopsis thaliana)和水稻(Oryza sativa)的APX同源性均在80%以上,并含有Ascorbate peroxidase保守结构域。系统发育分析结果显示,HbAPX属于过氧化酶体型APX。定量分析结果表明,该基因的表达受水杨酸(SA)的诱导,可能与植物的过敏反应(HR)和系统获得抗性(SAR)相关。Based on the sequencing results of RNA-Seq analysis of Hevea brasiliensis and the amino acid se- quence of APX1 ＂in Arabidopsis thaliana, the specific primers were designed and RT-PCR were performed to am- plify the APX cDNA of Hevea brasiliensis（HbAPX）. The results showed that the open reading frame of HbAPX is 858bp encoding 285 amino acids with a conserved ascorbate peroxidase domain ; the amino acid sequence of the HbAPX has 90% homology with APX from Jatropha curcas, and the homology is more than 80% with APX from Populus tomentosa, Gossypium hirsutum, Arabidopsis thaliana, Oryza sativa ; the phylogenetic analysis data indi- cated that HbAPX belong to peroxisome type APX; the quantitative analysis data suggested that the expression of HbAPX was induced by SA, which is probably involved in system acquired resistance and hypersensitivity reac- tion in rubber.

关 键 词：巴西橡胶树 抗坏血酸氧化物酶(APX) SA应答 

分 类 号：Q785[生物学—分子生物学]