牙龈卟啉菌菌毛基因在转基因番茄果实中的特异表达及小鼠免疫实验  

Expression of Porphyromonas Gingivalis Fimbrillin Gene in the Fruit of Transgenic Tomato and Its Immunization Assay

作　　者：戴海燕[1] 王华[2] 文少敏[1] 解娜[1] 邓芳成[1] 

机构地区：[1]海南医学院口腔医学院,海南海口570102 [2]海南医学院海南省热带病重点实验室,海南海口571101

出　　处：《热带生物学报》2011年第3期204-208,共5页Journal of Tropical Biology

基　　金：海南省自然科学基金资助项目(807096)

摘　　要：将编码牙龈卟啉菌菌毛蛋白(Fimbrillin,FimA)(第266～337Aa)与霍乱毒素B亚基(CTB)基因用农杆菌侵染法转入番茄植株,并利用番茄果实特异启动子E8使其在果实中实现特异表达,然后用特异表达的转基因番茄果实对实验小鼠进行口服免疫实验,研究转基因番茄果实的免疫原性。实验结果表明:获得的11株转化植株中,共有9株重组了CTB-FimA基因的植株在番茄果实中实现了特异表达;口服转基因番茄果实的免疫小鼠的血清中检测到抗FimA抗体;获得了以CTB为佐剂的在番茄果实中特异表达牙龈卟啉菌菌毛基因的植物口服疫苗候选植株。In this report, Agrobacterium-mediated transformation of tomato with plasmid pBIE8-CTB-FimA （266 -337 ） containing FimA and CTB gene with E8 promoter were performed, and the immunity of the trans- genie tomato fruit as an edible vaccine by oral immunization of mice were determined. The results indicated that the transgenic plants were confirmed by Southern blot and RT-PCR; the fusion proteins from leaf and fruit tissue of transgenic plants were detected by Western blot ; specific anti-FimA antibodies in the serum of mouse were de- tected, all of which suggested that candidate edible plant vaccine was obtained successfully.

关 键 词：番茄 牙周炎 FimA CTB E8启动子 

分 类 号：Q819[生物学—生物工程]