转基因水稻克螟稻标准物质的初制备  

Preparation of Reference Materials of Transgenic Rice KMD

作　　者：余笑波[1] 叶子弘[1] 隋志伟[2] 李亮[2] 臧超[2] 王晶[2] 

机构地区：[1]中国计量学院,浙江杭州310018 [2]中国计量科学研究院,北京100013

出　　处：《计量学报》2011年第B12期101-105,共5页Acta Metrologica Sinica

基　　金：国家转基因生物新品种培育科技重大专项（2008ZX08012003）;国家科技支撑计划项目（2008BAK41801）.

摘　　要：建立了转基因水稻克螟稻基体标准物质的初制备体系。按照0．5％、1％、2％、5％的质量比分别加入阳性粉末和阴性粉末，配制成4个水平的基体标准物质。实验结果表明，所选的阳性粉末和阴性粉末的水分分别为3．288％和4．039％，粒径均小于200斗m。从标识“金粉”的检测结果也可以看出，4个水平的基体标准物质均具有较好的均匀性。克螟稻基体标准物质的前期制备符合转基因基体标准物质的要求，可为后期进一步考察其稳定性和定值等参数提供基础。The preparation system of matrix reference materials transgenic rice KMD is established. 4 levels of matrix reference materials which contained KMD powder such as 0. 5% , 1% , 2% , 5% are prepared by mass ratio. The results show that, the water content of positive KMD powder and negative Xiushuill powder is 3. 288% and 4. 039% respectively and the diameter is less than 200μm. The detection result of labeling gold powder also show that 4 levels of matrix reference materials have well blended. The preparation system of reference materials of KMD is satisfied with matrix reference materials requirements and lay a foundation for the development in the detection and research of long stability and certified value.

关 键 词：计量学 基体 标准物质 转基因水稻 克螟稻 

分 类 号：TB99[一般工业技术—计量学]