转基因大豆Mon89788基体标准物质协同实验研究  

Cooperative Verification of the Value of GM Soybean Mon89788 Matrix Reference Materials

作　　者：张玲[1] 盛灵慧[1] 王晶[1] 曹应龙[2] 柳方方[3] 隋志伟[1] 卢长明[2] 

机构地区：[1]中国计量科学研究院,北京100013 [2]中国农业科学院油料作物研究所,湖北武汉430062 [3]北京化工大学,北京100029

出　　处：《计量学报》2011年第B12期106-109,共4页Acta Metrologica Sinica

摘　　要：对研制的3个含量水平的转基因大豆Mon89788基体标准物质进行多家实验室的验证研究。研究过程包括转基因大豆中基因组DNA的提取和转基因组分的定量PCR方法确认，标准物质的多家实验室协同实验，以及测最的不确定度评估。多家测定分析结果表明，转基因大豆Mon897883种不同外源基因／内源基因含量水平的标准物质参考值和扩展不确定度分别为0．35％±0．17％，0．61％±0．14％，3．17％±1．70％（k=2）。基体为大豆粉的该标准物质可用于转基因大豆含量的定量检测。Three levels of genetical]y modified soybeans Mon89788 matrix reference material are developed. Genetically modified soybean genomic DNA extraction and quantitative PCR of transgenic components methods are researched. A multi-laboratory verification and uncertainty evaluation are carried out too. The analysis of multi-laboratory value verification shows that in three different levels of genetically modified soybean Mon89788 reference materials, the transgenic gene / endogenous genes reference value and uncertainty is 0. 35% ± 0. 17% ,0. 61%± 0. 14% ,3.17% ± 1.70% （k = 2 ）, respectively. This set of reference materials that the matrix is soybean grand can be used for the quantitative detection of genetically modified soybeans content.

关 键 词：计量学 转基因大豆 Mon89788 实时荧光定量PCR 基体标准物质 

分 类 号：TB99[一般工业技术—计量学]