拟南芥转or基因突变体转录组及表型分析  

TRANSCRIPTOME AND PHENOTYPE ANALYSES OF or GENE TRANSGENIC ARABIDOPSIS MUTANT

作　　者：门潇[1] 孙天虎[1] 杨永华[1] 

机构地区：[1]南京大学生命科学学院,医药生物技术国家重点实验室,南京210093

出　　处：《北京师范大学学报（自然科学版）》2012年第1期47-53,共7页Journal of Beijing Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(30771167)

摘　　要：构建了拟南芥orange(or)过表达突变体和相应的对照组,通过比较它们在色素含量、转录组、表型等方面的变化,发现or在绿色组织(拟南芥叶子、茎等)中也能起到提高类胡萝卜素含量的作用,且突变体类胡萝卜素合成途径的基因转录水平没有显著变化,但是很多防卫胁迫相关基因转录水平上调,说明突变体中存在胁迫环境.对不同生长条件下突变体幼苗下胚轴的测量等表明or突变体对光尤其是蓝光变得十分敏感.本研究分析比较了or在不同组织中的效应,为or应用于改良作物类胡萝卜素含量的基因工程和进一步揭示or的作用机制提供了参考.It is known that orange（or） gene mutation leads to increased carotenoids content in plants.To elucidate the detailed mechanisms involved,Arabidopsis plants with over-expressed or and vector-only control were generated.Pigment content,transcriptome profile and mutant phenotypes were investigated.It was found that or was functional in green tissues,such as leaves and stems,although less functional than in non-green tissues.No major changes in transcription pattern were found for genes involved in carotenoids biosynthesis,but resistance-related genes,such as ZAT,were up-regulated,implying stressed environment in or mutants.Hypocotyl length measurements under different light conditions suggested that the or mutant became sensitive to light especially to blue light.Carotenoids enhancement of or in different plant tissues was analyzed,providing a reference for genetic engineering using or to improve nutritional status of crops,and for future work to uncover or regulatory mechanisms.

关 键 词：or基因 类胡萝卜素 转录组 胁迫 光表型 

分 类 号：S572.03[农业科学—烟草工业]