槭叶草离体快繁的研究  

Study on Rapid Propagation in vitro of Mukdenia rossii(Oliv.)Koidz

作　　者：王克凤[1] 赵春莉[1] 陈立飞[1] 赵和祥[1] 董然[1] 顾德峰[1] 

机构地区：[1]吉林农业大学园艺学院,吉林长春130118

出　　处：《北方园艺》2012年第5期135-136,共2页Northern Horticulture

基　　金：吉林省科学技术厅资助项目(20100259)

摘　　要：以槭叶草实生无菌苗为外植体,MS培养基为基础,通过添加不同种类和浓度的激素,筛选出槭叶草组织培养的最适培养基。结果表明:种子较好的灭菌时间为0.1%升汞6min;增殖的最适培养基为MS+6-BA 0.1g/L+NAA 0.1g/L,其增殖倍数高达5.1,并且苗生长状态良好;生根最适培养基为1/2MS。The experiment was based on MS culture media and Mukdenia rossii （Oliv.）Koidz. Used explants were from seedlings of the germination seeds in vitro. An appropriate culture medium was obtained via regulating the concentrations of cytokinin and auxin. The optimal sterilization method for seeds Mukdenia fossil （Oily.）Koidz was 1% HgCl2 6 rain. The propagation coefficient of 5. 1 times indicated that MS-ff6-BA 0. 1 mg/Lq-NAA 0. 1 nag/L was the optimal culture medium for subculture proliferation. The best rooting medium was 1/2MS. The survival rate of the plantlets was shown to be up to 97 %.

关 键 词：槭叶草 实生苗 组织培养 

分 类 号：S681.903.6[农业科学—观赏园艺]