仙茅中酚苷类成分高效液相色谱法测定方法的建立  

Simultaneous Determination of Phenolic Glycosides in Curculigins Rhizoma by HPLC

作　　者：汪滢[1,2] 刘雷[1,2] 徐金龙[2] 郭元晖[3] 秦路平[2] 张巧艳[1,2] 

机构地区：[1]福建中医药大学药学院中药学教研室,福州350108 [2]第二军医大学药学院生药学教研室,上海200433 [3]第二军医大学研究生管理大队药学队,上海200433

出　　处：《中国药学杂志》2012年第5期375-379,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81073115);上海市基础研究重点资助项目(09JC1417600)

摘　　要：目的建立同时测定仙茅中多种酚苷类化合物的HPLC方法。方法 Angilent 1100高效液相色谱仪,Zorbax SB C18色谱柱;检测波长:230 nm;柱温:30℃;进样量20μL;流速1 mL.min-1;流动相:乙腈(A)-0.1%醋酸水溶液(B),梯度洗脱程序为:0～25 min,4%～6%A;25～58 min,6%～17%A;58～85 min,17%～22%A。结果 8种酚苷成分5-羟甲基糠醛(1),2-羟基-5-(2-羟乙基)苯基-β-D-吡喃葡萄糖苷(2),苔黑酚龙胆二糖苷(3),苔黑酚葡萄糖苷(4),苔黑酚-1-O-β-D-呋喃芹糖基-(1→6)-β-D-吡喃葡萄糖苷(5),2,6-二甲氧基苯甲酸(6),仙茅苷(7)和仙茅素A(8)的质量浓度和峰面积呈良好的线性关系,平均加样回收率符合含量测定要求。结论该方法简便、灵敏、重复性好,适用于同时测定仙茅中多种酚苷类成分。OBJECTIVE To establish a RP-HPLC method for the simultaneous determination of phenolic glycosides in Curculigins Rhizoma. METHODS Angilent 1100 HPLC on a ZORBAX SB C18 column（4.6 mm×250 mm, 5 μm） with Extend C18 guard column（4.6 mm×12.5 mm, 5 μm） were used. The mobile phase consisted of acetonitrile（A）-0.1% phosphoric acid aqueous（B）. The gradient elution program was as follows： 0-25 min, 4%-6%A; 25-58 min, 6%-17%A; 58-85 min, 17%-22%A. The flow rate was kept at 1 mL·min-1, and the column temperature was set at 30 ℃. The detection wavelength was 230 nm. RESULTS The concentrations and peak areas of the 8 phenolic glycosides including 5-hydroxymethylfurfural（1）, 2-hydroxy-5-（2-hydroxyethyl）phenyl-β-D-glucopyranoside（2）, anacardoside（3）, orcinol glucoside（4）, orcinol-1-O-β-D-apiofuranosyl-（1→6）-β-D-glucopyranoside（5）, 2,6-dimethoxybenzoic acid（6）, curculigoside（7） and curculigine A（8） were in good linear relationship. The average recoveries of the 8 constituents met the requirement for determination. CONCLUSION The method is simple, sensitive, reproductive, and suitable for simultaneous determination of a variety of phenolic glycosides in Curculigins Rhizoma.

关 键 词：仙茅 酚苷类化合物 5-羟甲基糠醛 高效液相色谱法 多成分定量分析 

分 类 号：R284[医药卫生—中药学]