大鼠全血中靛玉红的含量测定及其药动学研究  

Determination of indirubin in rat whole blood and its pharmacokinetics study

作　　者：孟宪书[1] 郝小丽[1] 何淋[1] 黄罗生[1] 富志军[1] 

机构地区：[1]中国药科大学,南京211198

出　　处：《海峡药学》2012年第2期23-26,共4页Strait Pharmaceutical Journal

摘　　要：目的建立大鼠全血中靛玉红含量测定方法及大鼠体内靛玉红的药动学行为研究。方法采用超声、碱化及液．液萃取等方法处理全血样品；色谱条件：GLODSCl8（4．6×250mm，5um）色谱柱，流动相为甲醇．5％甲酸（68：32。v／v），流速：lmL·rain-1。检测波长289nm为检测条件；以5．6mg·kg-1的剂量大鼠尾静脉i．v．和200mg·kg。的剂量大鼠i．g．给药，采用非那雄胺为内标物，测定给药后全血中靛玉红的含量并用DAS2．1．1软件计算药动学参数。结果靛玉红在5．55—1110ng·mL-1的浓度范围线性关系良好，最低定量限为5．55ng·mL-1。，提取回收率71．8±6．496～80．7±6．596；大鼠灌胃和静脉注射给药后其体内药动学过程分属二室模型和三室模型．绝对生物利用度10．19％。结论实验表明本法操作简单，灵敏度高．重现性良好．结果准确可靠，可以用于大鼠体内靛玉红的药动学行为研究。OBJECTIVE To establish a HPLC method, to determined the content of indirubin in rat wholc blood sample, and study the pharmacokinetics activity of indirubin in vivo. METHODS The whole blood sampk was pro-processed by ultra-sonic extraction, alkalization and liquidliquid extraction. Chromatographic condition., were using a reverse-phase GI. ODS column（4.6 × 250mm, 5ttm）, with a mixture of methanol5 % formic acid（68 ： 32, v/v） as mobile phase at a flow rate of I. 0mL· rain- 1 and UV detection at 289nm. Pharmacokinetics stud3 was conducted by determination of indrubin in whole blood sample and calculated the pharmacokinetics parameters after i. v. and i. g. administration at dose of 5.6mg· kg- 1 and 200mg· kg- 1 respectively. RESULTS The calibra. tion curve of indirubin was linear at a range of 5.55 - 1110ng· mL-1. The lower limit of quantification wa： 5.55ng· mL-1. The extraction recovery was 71.8 ± 6.4 % -- 80.7 ± 6.5 %. The absolute bioavailability of indiru bin was 10.19 96. CONCLUSION The present HPLC method was specific, simple and sensitive for determinatiol of indirubin in rat whole blood sample and successfully estimated the pharmacokinetics parameters and bioavail ability of indirubin.

关 键 词：HPLC 靛玉红 大鼠 全血样品 药动学 

分 类 号：R969[医药卫生—药理学] R927.2[医药卫生—药学]