康肤霜对糖尿病大鼠烫伤创面愈合的作用  

Effect of Kangfu cream on the scald wound healing of rats with diabetes mellitus

作　　者：龚震宇[1] 童亚林[1] 万友华[2] 杨润生[3] 苏晓玲[3] 

机构地区：[1]中国人民解放军第181医院烧伤整形科,广西桂林541002 [2]中国人民解放军第181医院病理科,广西桂林541002 [3]中国人民解放军第181医院动物实验中心,广西桂林541002

出　　处：《重庆医学》2012年第7期679-682,共4页Chongqing medicine

基　　金：广西自然科学基金资助项目(2060203-255)

摘　　要：目的观察康肤霜对糖尿病大鼠深Ⅱ度烫伤创面愈合的影响。方法 Wistar大鼠24只,采用尾静脉注射四氧嘧啶法制成糖尿病大鼠模型。自动控时调压烫伤仪在大鼠背部制备4个深Ⅱ度烫伤创面,将创面随机分为康肤霜组、rhEGF组、聚维酮碘组及生理盐水组,分别采用康肤霜、rhEGF、聚维酮碘及生理盐水外敷,直至创面取材或创面愈合。观察创面愈合率及愈合时间。各组大鼠于创面治疗后第7、14、21天取创面组织,苏木素-伊红(HE)染色光学显微镜下观察创面组织病理学变化,链霉亲和素-生物素-过氧化物酶复合物(SABC)免疫组织化学法检测创面组织增殖细胞核抗原(PCNA)的表达,以标记指数法测定PCNA阳性细胞,评估组织创面的修复情况。结果康肤霜组大鼠创面愈合时间、愈合率均优于其他3组(P<0.05)。病理检查显示康肤霜能明显减轻创面早期的炎症反应,促进创面的再上皮化和表皮各层的分化。康肤霜组大鼠创面组织中PCNA的表达水平明显高于其他3组(P<0.05)。结论康肤霜能促进糖尿病大鼠烫伤创面细胞增殖,加速创面的愈合。Objective To observe the effects of Kangfu cream on deep Ⅱ degree burn wound healing of rats with diabetes mellitus.Methods Tail-vein injection of alloxan in 24 Wister rats were performed to establish diabetic rat models.Automatic time control and pressure regulation scald apparatus was employed to make 4 surfaces of deep Ⅱ degree burn wound on the back of rats.The wound surfaces were randomly divided into Kangfu cream group,rhEGF group,povidone-iodine group and physiological saline group in which rats were treated with Kangfu cream,rhEGF,povidone-iodine and physiological saline,respectively,until the wound tissue samples taken or the wound healing.The wound healing rates and the healing periods were observed.The samples of wound surface tissue of rats in each group were taken on the 7th,14th and 21th day after treatment.Hematoxylin-eosin（HE） staining was used to detect the pathological changes of wound tissue by optical microscope.Streptavidin biotin-peroxidase complex（SABC） immunohistochemistry was adopted to detect the expression of proliferating cell nuclear antigen（PCNA）,and the PCNA positive cells were measured by labeling index method to evaluate the tissue repair in wound surface.Results Both wound healing period and healing rates of rat in Kangfu cream group were superior to those in the other 3 groups（P0.05）.Pathological examination demonstrated that Kangfu cream could significantly reduce the early inflammatory response in wound surfaces,promote the wound re-epithelialization and the differentiation of each epidermal layer.The expression level of PCNA in wound surface tissue was markedly higher than those in the other 3 groups（P0.05）.Conclusion Kangfu cream can promote the cell proliferation in wound surface of rats with diabetes mellitus and facilitate the wound healing.

关 键 词：糖尿病 实验性 伤口愈合 增殖细胞核抗原 大鼠 

分 类 号：R644[医药卫生—外科学]