β-榄香烯对肿瘤细胞上清夜诱导的大鼠骨髓来源的内皮祖细胞增殖、管腔形成能力及细胞周期的影响  

Effect of β-elemene on proliferation,cell cycle and angiogenesis of endothelial progenitor cells

作　　者：宋颖[1,2] 孟晓[1] 杨向红[1] 刘云鹏[3] 

机构地区：[1]中国医科大学附属盛京医院病理科,沈阳110004 [2]河南省新乡医学院病理学教研室 [3]中国医科大学第一附属医院肿瘤科,沈阳110004

出　　处：《重庆医学》2012年第6期537-539,F0002,共4页Chongqing medicine

摘　　要：目的探讨β-榄香烯对内皮祖细胞(EPCs)参与肿瘤血管形成的抑制作用。方法原代培养大鼠骨髓来源内皮祖细胞,胃癌细胞株SGC-7901培养上清液刺激与β-榄香烯干预,四唑氮蓝还原反应(MTT)法检测内皮祖细胞增殖;碘化丙啶(PI)标记流式细胞仪检测细胞周期,Matrigel检测细胞管腔形成能力。结果β-榄香烯抑制肿瘤细胞上清液诱导的内皮祖细胞的增殖,并呈剂量和时间依赖性。细胞周期检测结果显示,β-榄香烯干预后,G1期细胞增多,进入S期及G2期细胞减少,细胞周期被阻滞于G1期;接种于Matrigel的内皮祖细胞形成管腔数目减少。结论β-榄香烯可抑制内皮祖细胞的增殖,阻滞其从G1期进入S期,并降低其形成管腔的能力。Objective To discuss the inhibitory action of β-elemene on endothelial progenitor cells（EPCs） angiogenesis.Methods EPCs were primarily cultured and treated with tumor cells supernatant and β-elemene;MTT assay was used to evaluate the proliferation of EPCs;the cell cycle was analyzed by using flow cytometry with PI staining;Matrigel was used to evaluate the angiogenic ability of EPCs.Results After treating with tumor cells supernatant and β-elemene,the proliferation of EPCs was apparently inhibited in a dose-dependent and time-dependent manner;FCM assays showed that EPCs in G1 phase were increased,while in S phase and G2 phase were decreased,which indicated that G1 phase arrest happened;EPCs after treating with tumor cells supernatant and β-elemene,were planted to Matrigel,and the number of tube formation was decreased notably compared with control group.Conclusion β-elemene can directly influence the proliferation and cell cycle of EPCs,arrest cells entering S phase from G1 phase and decrease their angiogenic ability.

关 键 词：Β-榄香烯 内皮祖细胞 肿瘤细胞上清液 细胞增殖 细胞周期 管腔形成能力 

分 类 号：R285.5[医药卫生—中药学]