干、湿化学检测系统测定肾功能高值标本时稀释液的选择与评价  

Choice and evaluation of different diluents for dectcting high-value samples of renal function items with dry and wet chemisty systems

作　　者：贺勇[1] 陈嵌 唐治贵[1] 刘容海[1] 唐佳[1] 高中燕[1] 米永华[1] 袁作为[1] 

机构地区：[1]重庆医科大学附属永川医院检验科,402160 [2]重庆市南川区人民医院检验科,408400

出　　处：《重庆医学》2012年第6期542-544,共3页Chongqing medicine

摘　　要：目的系统评价不同稀释液对干化学、湿化学检测系统测定肾功3项高值标本的影响,确定理想的稀释液。方法收集接近各系统肾功能3项检测上限的新鲜血清,分别用蒸馏水(DH2O)、生理盐水(NS)、7%小牛血清(7%BSA)、混合血清进行倍比稀释(最高稀释倍数为1∶32),在美国强生Vitros 350干化学分析仪和日本日立7600全自动生化分析仪上分别测定,然后进行偏差分析和线性评价。结果在湿化学检测系统上,尿素用NS、BSA、混合血清稀释,偏差分析均符合要求。肌酐、尿酸用DH2O、BSA、混合血清均符合要求。在干化学检测系统上,尿素、尿酸用DH2O和混合血清稀释均符合要求,肌酐除了BSA外各稀释液均符合要求。根据分析测量范围评价(AMR),在湿化学检测系统中,尿素、肌酐、尿酸用4种稀释液稀释后均符合要求;在干化学检测系统中,尿素只有DH2O和混合血清稀释后符合要求,肌酐只有NS和混合血清稀释后符合要求,尿酸用4种稀释液稀释后均符合要求。结论混合血清是最理想的稀释液。Objective To compare the results with different diluents on the high values of renal function test items by dry and wet chemisty systems and to find the ideal diluent.Methods Fresh serum samples with high concentration of renal function indexes including urea,creatinine（Cr） and uric acid（UA） were collected,which high value was close to the up limitation of the linear range,respectively.The samples of serum were diluted with distilled water（DH2O）,normal saline（NS）,BSA and mixed serum,respectively,and the highest dilutability was 1∶32 for each sample.All the samples were simultaneously tested by Qiangsheng Vitros 350 dry chemistry analyzer and Hitachi 7600 automatic biochemical analyzer.The results were analyzed by variance analysis and linear estimation.Results In wet chemistry system it was found that urea diluted with NS,BSA and mixed serum could meet the standard of quality assay,both Cr and UA diluted with DH2O,BSA and mixed serum could meet the standard.In dry chemistry system,both Urea and UA diluted by DH2O and mixed serum could meet the standard,Cr diluted with all diluents except BSA could meet the standard.For linear estimation：in wet chemistry system,urea,Cr and UA diluted by four diluents all could meet the standard;in dry chemistry system urea diluted with DH2O and mixed serum could meet the standard,Cr diluted with NS and mixed sernm could meet the standard,UA diluted with four diluents all met the standard.Conclusion Mixed serum is the ideal diluent.

关 键 词：稀释液 干、湿检测系统 尿素 肌酐 尿酸 

分 类 号：R446.1[医药卫生—诊断学]