Ⅰ类整合子在耐多药产ESBLs大肠埃希菌中的分布探讨  

Distribution of class Ⅰ integron in multi-drug resistant β-lactamases-producing strains of Escherichia coli

作　　者：李佩波[1,2] 蔡敏泓[1] 黄永茂[1] 林雁[1] 邹永胜[1] 钟利[1] 陈枫[1] 陈庄[1] 向成玉[1] 

机构地区：[1]泸州医学院附属医院感染科,四川泸州646000 [2]四川省绵阳市404医院/川北医学院第二附属医院感染科

出　　处：《重庆医学》2012年第6期547-549,共3页Chongqing medicine

基　　金：四川省重点学科重点建设项目(SZD0241)

摘　　要：目的了解耐多药产超广谱β-内酰胺酶(ESBLs)大肠埃希菌中Ⅰ类整合子携带情况。方法纸片扩散法(K-B法)测定大肠埃希菌对6种3类抗生素的敏感性并筛选出产ESBLs酶菌株;聚合酶链反应(PCR)检测整合子遗传标记qacE△1sul1。结果 71株大肠埃希菌中QacE△1-sul1共检出阳性菌株55株(74.46%),其中多耐模式检出率最高为88.89%(40株)。不同耐药模式中遗传标记基因的阳性率比较差异有统计学意义(P<0.05);产酶菌株在不同耐药模式中检出差异有统计学意义(P<0.05);非产酶菌株中检出差异无统计学意义(P>0.05)。结论耐多药产ESBLs大肠埃希菌中Ⅰ类整合子携带率高。Objective To understand the carrier status of class Ⅰ integron in multi-drug resistance β-lactamases-producing strains of Escherichia（E.） coli.Methods The disk diffusion test（K-B method） was used to detect the susceptibility of 71 strains of E.coli to 6 antibiotics and confirmed the ESBLs-producing strains.Polymerase chain reaction（PCR） was adopted to detect the genetic maeker qacE△1sul1 of class I integron.Results Among 71 strains of E.coli,55 strains（74.46%） were positive qacE△1sul1 of class I integron.The multi-drug resistant patern had the highest detection rate（88.89%,40 strains）.The difference in positive rates of genetic maker gene had statistical significce among different drug resistance patterns（P0.05）.The detection rate of different drug-resistance paterns had statistical difference in ESBLs-producing strains（P0.05）,while no significant difference in non-ESBLs-producing strains（P0.05）.Conclusion The ESBLs-producing strains carrying genetic markers of class Ⅰ Integron is existed mostly in resistant strains,especially in the multi-drug resistant pattern.

关 键 词：整合子类 Β内酰胺酶类 大肠埃希菌 耐多药 

分 类 号：R446.5[医药卫生—诊断学]