寡聚糖激发子对杨树防御酶系活性的影响  

Effects of the Activities of Defense Enzymes on Poplar by Oligosaccharides Elicitors

作　　者：李堆淑[1] 胡景江[2] 

机构地区：[1]商洛学院生物医药工程系,陕西商洛726000 [2]西北农林科技大学生命科学学院,陕西杨陵712100

出　　处：《西北林学院学报》2012年第2期26-29,43,共5页Journal of Northwest Forestry University

基　　金：国家"十五"科技攻关项目(2001BA501A09);陕西省教育厅2010年科学研究计划项目(2010JK522);陕西省商洛市2010年科学研究计划项目(SK12010-10)

摘　　要：采用3种寡聚糖(溃疡菌菌丝体、壳聚糖、果胶糖)激发子A1、B、C3分别诱导处理毛白杨愈伤组织,并在诱导48h后接种杨树溃疡病菌,测定体内4种病害防御酶系活性的变化。结果表明:毛白杨愈伤组织经这3种激发子处理后,其过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和多酚氧化酶(PPO)活性均明显高于对照(未经激发子处理而直接接种病菌的愈伤组织),从而证实了这几种激发子能诱导毛白杨愈伤组织对杨树溃疡病产生一定抗病性。Three oligosaccharides（mycelia of Dothiorella gregaria,chitosan-oligosaccharides and pectose-oligosaccharides） were used as elicitors to treat to the callus of Chinese white poplar（Populus tomentosa）.Dothiorella gregaria was inoculated the callus 48 h after the treatment of oligosaccharides.The changes of the activities of four defense enzymes were measured on callus of the poplar.The results indicated that the activities of POD,SOD,CAT and PPO of the three saccharide-treated calluses were higher than those of the control（nonsaccharide-treated calus）,indicating that the elicitors could induce P.tomentosa to produce resistance to D.gregaria.

关 键 词：寡聚糖 激发子 杨树溃疡病菌(Dothiorella gregaria) 毛白杨 愈伤组织 防御酶系 

分 类 号：S763.7[农业科学—森林保护学]