复合菌群产絮凝剂MAC37的特征及其在黏合剂废水中的应用  

Characteristics of MAC37 Produced by Multiple Bioflocculant-Producing Microorganisms and Its Application in Adhesive Wastewater

作　　者：苏晓梅[1] 张慧芳[2] 申秀英[1,3] 丁林贤[2] 横田明[4] 

机构地区：[1]浙江师范大学生化学院,中国浙江金华321004 [2]浙江师范大学地环学院,中国浙江金华321004 [3]浙江科技学院生物与化学工程学院,中国浙江杭州310023 [4]日本东京大学分子细胞生物学研究所

出　　处：《环境科学研究》2012年第3期340-345,共6页Research of Environmental Sciences

基　　金：浙江省大学生科技创新项目(2011R404014);浙江师范大学教授科研基金项目(ZC304009160)

摘　　要：以利用复活促进因子(Rpf)从土壤和污水处理系统中分离得到的菌种作为筛选絮凝剂产生菌的菌源,采用高岭土悬浊液为活性评价体系,筛选出4株絮凝率高于50%的菌株.经两两菌株复配,构建出产高效絮凝剂的复合菌群M3+M7,该菌群经优化培养后,絮凝率达96.27%.将其产生的絮凝剂进行提纯固化得絮凝剂粗品MAC37,对其主要成分进行定性和定量分析,并将复合菌群的发酵液应用于黏合剂废水的处理.结果表明:MAC37的主要成分为多糖和蛋白质,含量分别为74.5%和20.4%;黏合剂废水经复合菌群发酵液絮凝处理后,浊度、色度及CODCr的去除率分别为92.57%、94.73%和92.12%.Some bacteria were isolated from soil and sewage treatment systems by adding a resuscitation promoting factor （Rpf） to the culture medium. From these bacteria, four strains whose flocculation efficiency was higher than 50% were selected by adopting Kaolin suspension as the active evaluation system. Through a cuhure operation, multiple colonies of M5 ＋ M7 with high flocculation efficiency were constructed. Its flocculation efficiency could reach 96.27% under optimal conditions. The crude bioflocculant MAC37 was obtained by purification and solidification of the fermentation liquid cultured under optimized conditions, and the application of multiple microorganisms fermentation in adhesive wastewater treatment was studied. Qualitative and quantitative analysis showed that the main components of MAC37 were polysaccharides （74.5%） and proteins （20.4%）. The removal rate of turbidity, color and CODe, of the adhesive wastewater were 92.57% , 94. 73% and 92. 12% , respectively, after the treatment by the mhiple fermentation.

关 键 词：复活促进因子 复合菌群 MAC37 主要成分 黏合剂废水 

分 类 号：Q939.99[生物学—微生物学] X703[环境科学与工程—环境工程]