不同浓度的尼可地尔对心肌细胞膜K_(ATP)通道的影响  

The effect of different dose of K_(ATP) channel opener on the cardiac plasma membrane K_(ATP) channel nicorandil

作　　者：冯力[1] 刘伊丽[1] 刘杰[2] 邓英姿[1] 袁勇[1] 马立勤[1] 赖文岩[1] 

机构地区：[1]第一军医大学南方医院心内科,广东广州510515 [2]第一军医大学病理生理教研室,广东广州510515

出　　处：《第一军医大学学报》2000年第1期32-35,共4页Journal of First Military Medical University

摘　　要：本实验在急性分离的豚鼠心肌细胞上，用膜片钳技术单通道记录的内面向外记录模式研究了不同浓度的尼可地尔对细胞膜Ｋ_（ＡＴＰ）通道的影响。结果证实： ０．１ｍＭ的该药物不能激活Ｋ_（ＡＴＰ）通道，而１ｍＭ的药物可明显激活细胞膜Ｋ_（ＡＴＰ）通道。其电导为９２．７ ｐＳ，通道的开放时间常数τ_（Ｏ２）和开放概率较对照组（加入０．５ｍＭ　ＡＴＰ）明显增加，并可诱发通道出现二级开放。表明尼可地尔对心肌细胞膜Ｋ_（ＡＴＰ）通道呈剂量依赖性激活。Objective To study the effect on cardiac plasma membrane (CPM) K_ATP channel of isolated ventricular myocytes of different dose of K_ATP channel opener, nicorandil (NIC). Method Single channel patch clamp technique was used. Result it was showed that CPM K_ATP channel current could not be activated by 0. 1 mM NIC, but which could be remarkably induced by 1 mM NIC, and the conductance of K_ATP channel current activated by NIC was 92.7 pS. The slow time constant of the open time and the open state probability (Po) were increased compared with control group, and the secondary opening of K_ATP channel was induced by NIC, Conclusion NIC can affect CPM in a dose-dependent manner

关 键 词：尼可地尔 心肌细胞 KATP通道 豚鼠 

分 类 号：R965[医药卫生—药理学]