Wnt/β-catenin基因对骨髓瘤患者间充质干细胞成骨潜能的影响  

Effect of Wnt/β-catenin on osteogenic potential of mesenchymal stem cells from myeloma patients

作　　者：黄瑞宏[1,2] 王宁[1,2] 李祎[1,2] 张琼丽[1,2] 楼瑾[1,2] 李明[1,2] 杜新[1,2] 卓家才[1,2] 

机构地区：[1]广东省深圳市血液病研究所 [2]深圳市第二人民医院血液科,广东深圳518035

出　　处：《临床血液学杂志》2012年第2期160-162,168,共4页Journal of Clinical Hematology

基　　金：深圳市科技信息局科研基金资助项目(No:201002034)

摘　　要：目的:探讨Wnt/β-catenin基因和多发性骨髓瘤骨病的关系。方法:分离培养多发性骨髓瘤患者和正常人的骨髓间充质干细胞(MSCs),体外诱导分化成骨细胞,茜素红实验检测矿化物沉积、荧光定量RT-PCR方法检成骨指标(OPN、OC、ALP、Cbfal)和Wnt/β-catenin mRNA表达,分析MSCs细胞成骨能力变化及两组间Wnt/β-catenin mRNA表达差异。结果:骨髓MSCs体外成骨诱导后,茜素红染色阳性,呈现明显红色钙化结节;MSCs体外诱导成骨细胞,试验组与对照组比较,成骨指标OPN、OC、ALP、Cbfαl mRNA表达降低(P<0.05);骨髓MSCs体外成骨诱导后β-catenin mRNA表达降低(P<0.05)。结论:骨髓MSCs体外可成功诱导分化为成骨细胞;多发性骨髓瘤患者MSCs向成骨细胞分化潜能比正常人降低,Wnt/β-catenin可作为多发性骨髓瘤骨病潜在治疗靶点。Objective：To investigate the relationship between Wnt/β-catenin and multiple myeloma bone disease（MBD）.Method：Bone marrow mononuclear cells from MM and controls were isolated,cultured,expanded and then induced to osteogenic differentiation.Realtime Quantitative PCR was employed to detect the osteogenic markers,including Wnt/β-catenin,OPN,OC,ALP,Cbfαl.Alizarin red staining was conducted for detecting mineral deposition.The mRNA expressions of Wnt/β-catenin and osteogenic potential in the two groups were analyzed.Result：Positive alizarin red staining and the red calcium nodules appeared on the MSC post-osteogenic induction in vitro.The mRNA expressions of OPN,OC,ALP,and Cbfαl were significantly lower than those of control groups（P0.05）.The mRNA expression of β-catenin on the MSC post-osteogenic induction in vitro in the experimental groups was significantly lower than those of control groups（P0.05）.Conclusion：MSCs can be successfully induced to osteoblasts.The osteogenic potential of MSCs in MM patients is lower than controls.Wnt/β-catenin may present a novel target for the future therapy of MBD.

关 键 词：骨髓间充质干细胞 成骨细胞 实时聚合酶链反应 WNT/Β-CATENIN 

分 类 号：R331.2[医药卫生—人体生理学]