Effects of waterborne Fe(Ⅱ) on juvenile turbot Scophthalmus maximus:analysis of respiratory rate,hematology and gill histology  

作　　者：吴志昊 尤锋 刘洪军 刘梦侠 李军 张培军 

机构地区：[1]Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences [2]Shandong Provincial Mari-Culture Institute

出　　处：《Chinese Journal of Oceanology and Limnology》2012年第2期193-199,共7页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Key Technology R&D Program(Nos.2011BAD13B04,2006BAD09A11);Shandong Agricultural Seed Stock Breeding Project

摘　　要：The concentration of Fe（II） is high in some groundwater supplies used in turbot culture, and the toxicity of waterborne Fe（II） is unknown. We investigated the stress responses of juvenile turbot, Scophthalmus maximus, exposed to Fe（II） of different concentrations （0.01, 0.05, 0.1, 0.5, 1, and 2 mg/L） for 1, 7, 14, and 28 d, under the same ambient conditions of other parameters. Changes in respiratory rate, hematological parameters, and gill structure were determined. The results show that waterborne Fe（II） did not cause severe hematological perturbation to turbot. A low-medium Fe（II） concentration （lower than 0.1 mg/L） could boost the respiratory rate, and caused no or very limited damage to fish. A high Fe（II） concentration （0.1 mg/L or higher）, however, caused gill damage, such as vacuoles in branchial lamellae, epithelial necrosis, and hypertrophy of epithelial cells, and even death after extended exposure time. Therefore, excess waterborne Fe（II） and long-term exposure to Fe（II） could be responsible for poor growth and high mortality of turbot in culture. The concentration of waterborne Fe（II） in turbot culture should be kept below 0.1 mg/L.The concentration of Fe(Ⅱ) is high in some groundwater supplies used in turbot culture,and the toxicity of waterborne Fe(Ⅱ) is unknown.We investigated the stress responses of juvenile turbot,Scophthalmus maximus,exposed to Fe(Ⅱ) of different concentrations (0.01,0.05,0.1,0.5,1,and 2 mg/L) for 1,7,14,and 28 d,under the same ambient conditions of other parameters.Changes in respiratory rate,hematological parameters,and gill structure were determined.The results show that waterborne Fe(Ⅱ) did not cause severe hematological perturbation to turbot.A low-medium Fe(Ⅱ) concentration (lower than 0.1 mg/L) could boost the respiratory rate,and caused no or very limited damage to fish.A high Fe(Ⅱ) concentration (0.1 mg/L or higher),however,caused gill damage,such as vacuoles in branchial lamellae,epithelial necrosis,and hypertrophy of epithelial cells,and even death after extended exposure time.Therefore,excess waterborne Fe(Ⅱ) and long-term exposure to Fe(Ⅱ) could be responsible for poor growth and high mortality of turbot in culture.The concentration of waterborne Fe(Ⅱ) in turbot culture should be kept below 0.1 mg/L.

关 键 词：Scophthalmus maximus waterborne Fe（II） respiratory rate hematological parameter gill structure 

分 类 号：S917.4[农业科学—水产科学]