Single nucleotide polymorphisms in intron 1 and intron 2 of Larimichthys crocea growth hormone gene are correlated with growth traits  

作　　者：倪静 尤锋 许建和 徐冬冬 文爱韵 吴志昊 徐永立 张培军 

机构地区：[1]Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences [2]Key Laboratory of Marine Biotechnology of Jiangsu Province,Huaihai Institute of Technology [3]Key Laboratory of Mariculture and Enhancement,Marine Fishery Institute of Zhejiang Province

出　　处：《Chinese Journal of Oceanology and Limnology》2012年第2期279-285,共7页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Basic Research Program of China(973Program)(No.2010CB126304);the Special Fund for Agro-scientific Research in the Public Interest(No.200903046);the Project from the Ministry of Science and Technology of China(No.2006DKA30470017)

摘　　要：The growth hormone gene (GH) affects animal growth and is a potential target for genetic studies of variation related to growth traits. In this study, we analyzed single nucleotide polymorphisms (SNPs) in GH intron regions and their associations with growth traits in large yellow croaker, Larimichthys crocea, from Zhejiang and Fujian stocks. The results of PCR-single strand conformation polymorphism showed two haplotypes of intron 1, named AA and AB genotypes, in Zhejiang stock. AB exhibited an SNP at position 196 (G A) that was negatively correlated with body height and positively correlated with standard length/body height (P 0.05). Two different genotypes, CC and CD, were identified in intron 2 in Fujian stock, with CD showing an SNP at position 692 (T C). The CD genotype had a significantly positive correlation with both weight and total length (P 0.01). These basic data highlight the potential for using GH as a genetic marker of fish growth in marker assisted selection.The growth hormone gene （GH） affects animal growth and is a potential target for genetic studies of variation related to growth traits. In this study, we analyzed single nucleotide polymorphisms （SNPs） in GH intron regions and their associations with growth traits in large yellow croaker, Larimichthys crocea, from Zhejiang and Fujian stocks. The results of PCR-single strand conformation polymorphism showed two haplotypes of intron 1, named AA and AB genotypes, in Zhejiang stock. AB exhibited an SNP at position 196 （G→A） that was negatively correlated with body height and positively correlated with standard length/body height （P≤0.05）. Two different genotypes, CC and CD, were identified in intron 2 in Fujian stock, with CD showing an SNP at position 692 （T→C）. The CD genotype had a significantly positive correlation with both weight and total length （P≤0.01）. These basic data highlight the potential for using GH as a genetic marker of fish growth in marker assisted selection.

关 键 词：large yellow croaker （Larimichthys crocea） growth hormone gene （GH） PCR-single strandconformation polymorphism （PCR-SSCP） single nucleotide polymorphism （SNP） growth trait 

分 类 号：S917.4[农业科学—水产科学]