应用3种方法检测鼠疫恢复期患者血清中F1抗体水平  

Detection of F1 antibody levels in convalescent sera from plague patients with three assays

作　　者：杜春红[1] 王鹏[1] 梁云[1] 唐雪[1] 张洪英[1] 洪梅[1] 宋志忠[1] 

机构地区：[1]云南省地方病防治所云南省鼠疫防控技术重点实验室,云南大理671000

出　　处：《疾病监测》2012年第2期145-147,共3页Disease Surveillance

基　　金：云南省科技攻关项目(No.2007CA010)~~

摘　　要：目的了解鼠疫恢复期患者血清F1抗体水平的维持情况及间接血凝、酶联免疫、胶体金免疫层析技术在鼠疫F1抗体检测中的应用。方法应用间接血凝试验(IHA)、双抗原夹心酶联免疫吸附试验(DAgs-ELISA)和胶体金免疫层析试验(GⅠCA)3种方法,对比检测25例鼠疫恢复期患者血清中F1抗体。结果 25份血清样品F1抗体IHA阳性19份,DAgs-ELISA阳性21份,GⅠCA阳性18份。F1抗体滴度:IHA 15例为1∶80～1∶320;ELISA 10例为1∶640～1∶1280。结论大部分鼠疫恢复期患者血清能检出F1抗体,3种F1抗体检测方法均为敏感、特异的检测技术。Objective To understand the duration of F1 antibodies in the sera from plague patients in convalescent period and the effectiveness of three detecting assays of indirect hemagglutination test （ IHA）, double-antigen sandwich enzyme-linked immunosorbent assay （ DAgs-ELISA ） and colloidal gold immunochromatography （ GICA ）. Methods Twenty five serum samples from plague patients in convalescent period were detected with IHA, DAgs-ELISA and GICA, respectively. Results Among the 25 serum samples, 19, 21 and 18 were detected to be positive in IHA test, DAgs-ELISA and GICA, respectively. F1 antibody titers were 1：80 - 1：320 in 15 positive samples in IHA test, and 1：640 -1：1280 in 10 positive samples in DAgs-ELISA. Conclusion F1 antibodies could be detected in convalescent sera in most plague patients by conducting three assays above mentioned, indicating they are sensitive and specific in plague detection.

关 键 词：鼠疫F1抗体 恢复期患者血清 检测方法 

分 类 号：R516.8[医药卫生—内科学]