免抗流脑菌Fab'-HRP交联物的制备与鉴定  

PREPARATION AND IDENTIFICATION OF Fab'-HRP CONJUGATE

作　　者：李宁华[1] 胡真[1] 李爱芳[1] 陈惠黎[2] 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所 [2]上海第一医科大学生物化学教研室

出　　处：《中国公共卫生学报》1990年第3期183-186,共4页

摘　　要：通过硫酸钠—DEAE纤维素法,胃酶消化法及流基乙醇还原法制备了免抗A群流脑菌Fab’片断。所得到的Fab’片断通过一种异双功能基因交联剂HECCM与HRP连接,成功制备了Fah’-HRP交联物。后者通过凝胶过滤层析鉴定,分子量为86099。该交联物用于ELISA双抗体夹心法检测流脑A群菌标准抗原,敏感度达0.225ng/ml。Fab' fragments was prepared using sodium su- fate-DEAE cellulose chromatography, digestion w- ith pepsin and reduction with 2-mercaptoenthnol. Then the HRP was cross-linked to Fab' through HECCM to form Fab'-HRP conjugate. The MW of the conjugate was found to be 86,099 with gel filt- ration chromatography. Fab'-HRP conjugate was applied to sandwich ELISA and the Fab'-ELISA was established. The sensitivity of the method to detect APS was 0. 225ng/ml.

关 键 词：免抗流脑菌 Fab-HRP 交联物 

分 类 号：R392－33[医药卫生—免疫学]