HPLC-MS/MS法测定大鼠血浆中LH14的含量  

Assay for LH14 by HPLC-MS/MS in Rat Plasma

作　　者：王彬[1,2] 杨雷琼[2] 吴卓娜[2] 顾若兰[2] 朱晓霞[2] 孟志云[2] 甘慧[2] 

机构地区：[1]解放军总医院检验科,北京100853 [2]军事医学科学院野战输血研究所,北京100850

出　　处：《解放军药学学报》2012年第1期38-40,44,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助项目;No.NSFC20903118

摘　　要：目的建立SD大鼠血浆中LH14的测定方法。方法以CS55为内标,采用HPLC-MS/MS法测定,以Ulti-mate C18色谱柱(50 mm×2.1 mm,3μm)为分析柱,甲醇-水为流动相,梯度洗脱,流速为0.2 ml/min,柱温为25℃,进样量5μl。结果在2～500 ng/ml浓度范围内具有良好的线性关系,线性方程为:Y=0.0333+0.0043X,R2=0.9928(n=8)。精密度与准确度均符合生物样品的测定要求,最低定量浓度为2 ng/ml。结论该检测方法简便、准确、专属性强,能够满足LH14在SD大鼠体内药代动力学研究的需要。Objective To develop a method for the determination of LH14 in plasma of SD rats.Methods CS55 was used as the internal standard.LH14 was chromatographed on Thermo-Finnigan TSQ Quantum with an Ultimate columnC18（50 mm×2.1 mm,3 μm）.The mobile phase was methanol-water at a flow rate of 0.2 ml/min.The column temperature was 25℃ and the injection volume was 5 μl.Results A good linear relationship was obtained between the peak area ratio of LH14/CS55 and the concentration of LH14 over the range of 2-500 ng/ml.The linear equation was Y=0.0333＋0.0043X,R2=0.9928（n=8）.The limit of quantitation was 2 ng/ml.Conclusion This method is simple,quick,accurate and suitable for the pharmacokinetic study of SD rats.

关 键 词：LH14 HPLC-MS/MS 药代动力学 

分 类 号：R969.1[医药卫生—药理学]