HPLC法测定兔血浆中茜草双酯的药物浓度  

Determination of Rubidate in Rabbit Plasma by Reversed Phase HPLC

作　　者：陈莉[1] 肖若蕾[1] 

机构地区：[1]咸宁学院药学院,湖北咸宁437100

出　　处：《解放军药学学报》2012年第1期49-51,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立兔血浆中茜草双酯的血药浓度测定方法。方法采用HPLC法:Eurospher100-5 C18色谱柱(250 mm×4.6 mm,5μm);流动相为磷酸(0.05 mol/L,二乙胺调pH值至5)-乙腈(30∶70);流速为1.0 ml/min;检测波长为264 nm。结果茜草双酯在0.084～20.08μg/ml浓度范围内线性关系良好;低,中,高浓度的平均回收率分别为:86.9%、89.3%、94.2%。结论该法简便,灵敏,可靠,可用于茜草双酯血药浓度检测。Objective To establish a method to determine the content of rubidate in rabbit plasma by HPLC.Methods HPLC was used to determine the content of rubidate in rabbit plasma.The separation was performed on Eurospher100-5 C18（250 mm×4.6 mm,5 μm）column with phosphate（0.05 mol/L）：acetonitrile（30∶ 70） as the mobile phase.The flow rate was 1.0 ml/min and the wavelength was UV 264 nm.Results The liner response ranged from 0.084 to 20.08 μg/ml.The average recovery of low,medium and high concentrations was 86.9%,89.3% and 94.2%,respectively.Conclusion The method is simple,sensitive,reliable,which can be used for determination of rubidate in rabbits plasma and its pharmacokinetic study.

关 键 词：茜草双酯 HPLC 血药浓度 含量测定 

分 类 号：R927.2[医药卫生—药学] R969.1