戊型肝炎病毒实验感染动物研究  

Experimental Study of Infection of Rhesus Monkeys with Hepatitis E Virus

作　　者：王光明[1] 庄辉[2] 方喜业[1] 李凡[2] 李革[1] 李倬 关崇谦 沈洁[1] 郭晓霞[2] 冯启[2] 刘崇柏[4] 曹学义[5] 

机构地区：[1]中国医学科学院实验动物研究所 [2]北京医科大学 [3]北京第二传染病院 [4]中国预防医学科学院病毒学研究所 [5]新疆维吾尔自治区卫生防疫站

出　　处：《中国公共卫生学报》1990年第5期287-289,296,共4页

基　　金：卫生部<七;五>科技攻关课题之一

摘　　要：选用1～3岁龄的猕猴(maca mulatta)15只(其中有2只对照)进行了戊型肝炎病毒(Hepatitis E virus简称HEV)的感染实验。第1代感染两批动物:第1批感染4只,3只发病;第2批感染4只,全部发病。后用第2批感染猴的粪便及肝脏悬液作为第1代,进行第2代感染。第2代感染4只猴,也全部发病,用肝悬液传代的1只猴也发病。感染后发病的猴血清丙氨酸转氨酶(ALT)水平均有不同程度的上升,一般高于正常值3～6倍,最高可10倍左右。肝活检可见肝细胞炎症和坏死。猴的粪便标本用免疫电镜(IEM)检测可见直径为27～34nm的病毒颗粒。An experimental model of hepatitis E was es- tablished in rhesus monkeys (Macaca mulatta). First-passage animals were inoculated with two different stool samples obtained from patients with hepatitis E which originated from Xinjiang and Shenyan, respectively. Three of four monkeys were infected. 27～34nm diameter viruslike particles were found in feces of infected animals by immune electron microscopy (IEM). Liver biopsy of the three monkeys showed that histopath ologic changes of actue-phase hepatitis. Secondpassage animals were inoculated with HEY in both stool and liver suspensions from a first-passage monkey (M-16), including one animal inoculated with liver suspension. All of them were infected.

关 键 词：戊型肝炎 戊型肝炎病毒 感染 

分 类 号：R512.03[医药卫生—内科学]