Sephadex G-25对乳酸链球菌素溶液脱盐的研究  

Study on desalting of nisin solution with sephadex G-25

作　　者：陈娟[1] 郑玉果[1] 赵波[1] 许炜[1] 赵文杰[1] 朱宝泉[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中国抗生素杂志》2012年第3期212-215,共4页Chinese Journal of Antibiotics

基　　金：微生物药物技术创新与新药创新产学研联盟;国家科技重大专项资助(No.2010ZX09401-403)

摘　　要：目的研究乳酸链球菌素的凝胶过滤脱盐法。方法比较在Sephadex G-25柱上加样量、加样体积、洗脱流速和高径比对脱盐效果的影响。结果确定了乳酸链球菌素的最佳脱盐工艺为:色谱柱高径比15:1,加样体积为每毫升凝胶0.1mL料液、加样量为每毫升凝胶7×104IU、用pH3.45的醋酸缓冲溶液在0.6mL/min流速下洗脱。在此条件下,乳酸链球菌素的脱盐收率和回收率都在90%以上。结论 Sephadex G-25能有效的将乳酸链球菌素和盐分离。Objective To study the process of gel filter desalination of nisin. Methods The sample load, sample volume, flowing rate and the ratio of height to diameter of Sephadex G-25 bed on the effect of desalination were compared. Results The optimum conditions for desalination of nisin： sample load 7×10^4 IV per milliliter gel, the sample volume 0. 1 mL/mL gel, the ratio of height to diameter of bed 15： 1, with flow fate of pH3.45 acetic acid buffer solution 0.6mL/min. On this condition, the desalination yield and recovery of nisin were both above 90%. Conclusion Sephadex G-25 can separate nisin and salt effectively.

关 键 词：乳酸链球菌素 SEPHADEX G-25 脱盐 

分 类 号：R978[医药卫生—药品]