外用含汞制剂拔毒生肌散诱导肾早期损伤的敏感监测指标的比较研究  

Comparative study on external use of mercury-containing preparation Badu Shengji San in sensitive monitoring indicators of induced early renal injury

作　　者：路艳丽[1] 贺蓉[1] 徐启华[1] 彭博[1] 李建荣[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2012年第6期706-710,共5页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2008BAI53B08)

摘　　要：目的:比较含汞制剂拔毒生肌散外用,对破损皮肤大鼠肾早期损伤指标尿β-N-乙酰氨基葡萄糖苷酶(NAG),β2-微球蛋白(β2-MG),视黄醇结合蛋白(RBP),簇集蛋白(CLU)的敏感性。方法:以含汞中药外用制剂拔毒生肌散(BaduShengji San)为汞接触药物,选择最低毒性剂量,观察其对破损皮肤大鼠肾脏系数、肾脏病理组织形态学、血清肌酐(SCr)、尿素氮(BUN)及尿肾早期损伤指标NAG,β2-MG,RBP,CLU的影响,并比较检测指标的敏感性。结果:与破损皮肤对照组比较,在拔毒生肌散60,120 mg.kg-1组大鼠肾脏系数明显升高、肾小管出现明显病理性改变时,血清SCr,BUN未见明显改变,但尿CLU含量呈升高趋势,尿NAG,RBP含量明显升高,且RBP可在拔毒生肌散30 mg.kg-1组大鼠肾小管未见明显病理改变时出现明显升高。但拔毒生肌散30,60 mg.kg-1组大鼠尿β2-MG含量明显下降,120 mg.kg-1剂量组大鼠尿β2-MG含量未见明显改变。结论:与SCr,BUN相比,尿RBP,β2-MG,NAG,CLU等作为肾早期损伤的监测指标更为敏感,其敏感性依次为RBP>NAG>CLU;尿RBP,NAG的升高比尿β2-MG的升高出现时间更早。Objective： To compare the sensitivity of early renal injury induced by mercury-containing medicine in rats, including urinary N-acetyl-β-D-glucosdminidase （NAG）, β2-mieroglobulin （β2-MG）, retinol binding protein （RBP） and clusterin （CLU）. Method： Badu Shengji San（ BDSJS）, a mercury-containing preparation of traditional Chinese medicine, was adopted as the mercury contact drug. The lowest effective toxic dose was used to observe its effect on serum creatinine （SCr） , blood urea nitrogen （BUN）, and such early renal injury indicators as NAG, RBP,β2-MG and CLU and compare the sensitivity of tested indicators. Result ： Compared to the broken skin group, groups with administration of 60 and 120 mg · kg-1 doses of BDSJS showed no obvious difference in SCr and BUN when kidney indicators is remarkably increased and obvious pathological changes were found in kidney tubules but with significant increase in the urinary level of CLU and the levels of NAG and RBP. H＆E staining of renal tubule showed that exposure of 30 mg· kg-1 BDSJS had no significant morphological changes, but at the same concentrations, the level of RBP was markedly increased. Urinary β2-MG levels were markedly decreased in BDSJS 30, 60 mg · kg-1 group rats, whereas 120 mg· kg-1 dose group showed no obvious change in urinary β2-MG levels. Conclusion： Urinary RBP, NAG and CLU were more sensitive than SCr and BUN as indicators for early renal injury in the order of RBP 〉 NAG 〉 CLU, and urinary RBP, NAG would increase earlier than β2-MG .

关 键 词：拔毒生肌散 破损皮肤大鼠 肾早期损伤 汞 RBP NAG Β2-MG CLU 

分 类 号：R285[医药卫生—中药学]