百蕊草种子休眠的破除与生理变化研究  

Study on seeds dormancy release and physiological change of Thesium chinense

作　　者：罗夫来[1,2] 郭巧生[1] 

机构地区：[1]南京农业大学中药材研究所,江苏南京210095 [2]贵州大学农学院,贵州贵阳550025

出　　处：《中国中药杂志》2012年第6期760-763,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173482);国家科技基础条件平台项目(2005DKA21000)

摘　　要：目的:探讨破除百蕊草种子休眠的方法,研究休眠破除前后百蕊草种子生理变化。方法:采用GA3处理与低温层积相结合的方法破除百蕊草种子休眠,测定种子中总糖、可溶性糖和粗脂肪含量,用HPLC测定内源GA3与ABA含量。结果:用流水冲洗24 h,再用500 mg.L-1的GA3溶液浸种24 h,然后低温层积处理150 d,百蕊草种子裂壳率达22%,种子层积后粗脂肪含量减少了50%以上,总糖和可溶糖增加了3～4倍,ABA减少了90%以上,GA3则增加了5倍以上。结论:用流水冲洗24 h,再用500 mg.L-1的GA3溶液浸种24 h,然后低温层积处理150～180 d可有效破除百蕊草种子休眠。Objective： To study the seeds dormancy release and physiological change of Thesium chinense. Method： To re- lease dormancy, the seeds of T. chinense were treated with chemical reagent and stratification under 3-5℃. Result： When washed with flowing water for 24 h, then soaked in 500 mg . L-1 GA3 for 24 h, finally, treated with stratification method under 3-5 ℃ for 150 day, the split rate of T. chinense seeds reached 22% , crude fat decreased 50% , total sugar and dissolvabihty sugar increased 3-4 times, ABA decreased more than 90% , GA3 increased more than 5 times. Conclusion： The seeds dormancy of T. chinense can be released with the method of washing with flowing water for 24 h, then soaking in 500 mg . L -1 GA3 for 24 h, finally, treated with stratification method under 3-5 ℃ for 150-180 day.

关 键 词：百蕊草 种子 休眠 

分 类 号：S567.239[农业科学—中草药栽培]