川木香煨制前后主要药效成分在人工胃、肠液及大鼠离体胃肠共孵液中的稳定性研究  

Study on stability of major pharmaceutical ingredients of Vladimiriae Radix before and after being roasted in artificial gastric juice,artificial intestinal juice and isolated rat gastric,intestinal or colonic incubation juice

作　　者：毛茜[1] 傅超美[1] 胡慧玲[1] 王战国[2,3] 何瑶[1] 包希福 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137 [2]四川大学生命科学学院,四川成都610064 [3]四川省茂县羌医药研究所,四川茂县623200

出　　处：《中国中药杂志》2012年第6期785-789,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年基金项目(81001638);四川省科技厅科技支撑计划项目(2011SZ0063)

摘　　要：目的:考察川木香煨制前后木香烃内酯(costunolide,COS)和去氢木香内酯(dehydrocostus lactone,DEH)在人工胃、肠液中的稳定性,及其在大鼠离体胃、小肠、结肠共孵液中的吸附、摄取或降解特征。方法:采用高效液相色谱法测定川木香煨制前后COS和DEH在人工胃、肠液及大鼠离体胃肠共孵液中的质量浓度,以大鼠离体胃、小肠、结肠与药物共孵的方法考察其物理吸附、摄取或降解参数。结果:川木香煨制前后COS在人工胃液稳定性较差,平均降解常数分别为0.758 0,0.531 1,煨制后稳定性有所提高,具统计学意义(P<0.01);川木香煨制前后COS和DEH在大鼠离体胃、小肠和结肠中的吸附、摄取或降解量(2 h)均较大,其中煨制前后COS和DEH在不同部位均有不同程度的差异。结论:川木香煨制前后COS在人工胃液中均不稳定,煨制后稳定性有所提高;大鼠离体胃、小肠、结肠对川木香煨制前后的COS和DEH均有明显的吸附、摄取或降解,并在不同部位有差异;为川木香煨制前后药效成分的吸收机制研究提供了参考。Objective： To study the stability of costunolide （COS） and dehydrocostus lactone （DEH） of Vladimiriae Radix before and after being roasted in artificial gastric juice, artificial intestinal juice and isolated rat gastric, intestinal or colonic incubation juice. Method： The HPLC method was used for the determination of the mass concentration of COS and DEH Vladimiriae Radix before and after being roasted artificial gastric juice, artificial intestinal juice and isolated rat gastric, intestinal or colonic incubation juice. The samples were incubated with isolated rat stomach, small intestine; colon was used to study physical adsorption, absorption or degradation parameters. Result： COS of Vladimiriae Radix before or after being roasted was unstable in artificial gastric juice, with the average degradation constants as 0. 758 0 and 0. 531 1. Having been roasted, it showed an increasing stability with a significant difference （P 〈 0. 01 ）. both of COS and DEH of Vladimiriae Radix before or after being roasted showed high adsorption, uptake or degradation （2 h） , and it had significant difference between different parts. Conclusion： COS was unstable in artificial gastric juice （unprocessed Vladimiriae Radix has a higher degradation rate）. Isolated rat stomach, small intestine, colon can adsorb, take, degrade COS and DEH of Vladimiriae Radix before or after roasting process obviously and differently. It provides basis for studies on the absorption mechanisms of effective ingredients of Vladimiriae Radix before and after being roasted.

关 键 词：川木香 煨制 木香烃内酯 去氢木香内酯 高效液相色谱法 稳定性 

分 类 号：R285[医药卫生—中药学]