半夏醇制减毒新方法研究(Ⅰ)——新方法及新工艺研究探讨  

Study on new toxicity-reducing methods of Pinellia Rhizoma prepared by ethanol(Ⅰ)-new methods and technology

作　　者：陶文婷[1] 郁红礼[1,2,3] 吴皓[1,2,3] 朱法根[1] 邱韵萦[1] 赵腾斐[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210029 [2]江苏省中药炮制重点实验室,江苏南京210029 [3]国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210029

出　　处：《中国中药杂志》2012年第6期790-795,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30772785,30973939);国家“重大新药创制”科技重大专项(2009ZX09308-004);江苏省普通高校研究生创新计划

摘　　要：目的:探索半夏醇制减毒新方法和最佳工艺参数。方法:以乙醇润制半夏减毒,采用正交试验设计考察乙醇量、润制时间、乙醇浓度、润制温度4个影响因素,以总游离有机酸含量为化学指标,以大鼠腹腔渗出液中PGE2含量为毒理指标,综合评分,以家兔眼结膜刺激性试验得分作为减毒的直观验证,优选半夏醇制减毒的最佳工艺,并以药效验证考察醇制工艺的合理性。结果:半夏最佳醇制工艺为生半夏在60℃,用0.5倍量的75%乙醇润制4 d,干燥后即得。半夏醇制后保留了半夏的镇咳、祛痰、抗炎作用。结论:该方法简单、可行,可作为半夏减毒存效新方法的研究方向。Objective：To explore new toxicity-reducing methods of Pinellia Rhizoma prepared by ethanol and the latest technical parameters. Method： Pineilia Rhizoma is prepared with ethanol. The orthogonal experimental design was adopted for investigating amount of ethanol, preparing time, ethanol concentration and preparing temperature. The optimal technology was determined by the comprehensive score of toxicological indicators of PGE2 content of rat celiac percolate, with the rabbit conjunctival irritation test as the intuitive validation on toxicology reduction. The pharmacodynamics validation was used to determine the reasonability of the preparation process. Result： The optimal technology was that Pinellia Rhizoma was prepared by 75% ethanol at the temperature of 60℃ by 4 days, and then dried. The effect of relieving cough, reducing sputum and anti-inflammatory of Pinellia Rhizoma is not reduced after prepared by ethanol Conclusion： The optimal technology of Pineilia Rhizoma prepared by ethanol is simple and reasonable that it can be used as the new method to reduce toxicity and keep efficacy of Pinellia Rhizoma.

关 键 词：半夏 醇制 减毒存效 正交试验 

分 类 号：R283[医药卫生—中药学]