HPLC同时测定山楂提取物中绿原酸和牡荆素鼠李糖苷的含量  

Simultaneous determination of chlorogenic acid and vitexin 2″-rhamnoside in Crataegi Fructus extracts by HPLC

作　　者：何雅君[1] 苏娟[2] 杨茜[2] 刘慧[2] 张卫东[1,2] 

机构地区：[1]上海交通大学药学院,上海200240 [2]第二军医大学药学院,上海200433

出　　处：《中国中药杂志》2012年第6期829-831,共3页China Journal of Chinese Materia Medica

基　　金：国家药典委员会标准研究项目(TS-46)

摘　　要：目的:采用HPLC建立山楂提取物中绿原酸和牡荆素鼠李糖苷的含量测定方法。方法:SHISEIDO CAPCELLPAK C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇(A)、四氢呋喃-乙酸-水(10∶2∶76,B),梯度洗脱,检测波长330 nm,流速1.0 mL.min-1,柱温25℃。结果:绿原酸和牡荆素鼠李糖苷分别在1.34～164.8,1.18～148.7 mg.L-1线性关系良好;平均加样回收率分别为100.4%,98.83%,RSD 1.5%,1.3%。结论:该方法简单、准确、重复性好,可用于测定山楂提取物中绿原酸和牡荆素鼠李糖苷的含量,为山楂提取物及其制剂的质量控制有效的方法。Objective： To establish a determination method for contents of chlorogenic acid and vitexin 2＂-rhanmoside in Crataegi Fructus extracts by HPLC. Method： The chromatographic column was SHISEIDO CAPCELL PAK Cla （4. 6 mm× 250 mm, 5 um） ; mobile phase was methanol（A） and THF-acetic acid-water （10： 2： 76, B）, with gradient elution, flow speed was 1.0 mL . min-1 ; detection wavelength was 330 nm; temperature of column was 25 ℃. Result： Chlorogenic acid and vitexin 2＂-rhamnoside showed good linear relationships within the ranges of 1.34-164. 8 and 1.18-148. 7 mg . L-1 , with the recovery rates being 100. 4% and 98. 8%, and RSD being 1. 5% and 1.3% respectively. Conclusion： The present method is so simply, accurate and highly repeatable that it can be used to effectively determine the contents of chlorogenic acid and vitexin 2＂-rhamnoside in Crataegi Fructus extracts.

关 键 词：山楂提取物 绿原酸 牡荆素鼠李糖苷 质量控制 HPLC 

分 类 号：R284[医药卫生—中药学]