紫金砂药材的指纹图谱研究  

Fingerprint of Angelica polymorpha by HPLC

作　　者：卢金清[1] 王琴[1] 许家琦[1] 何冬黎[1] 阮小云[1] 胡晓燕[1] 

机构地区：[1]湖北中医药大学,湖北武汉430065

出　　处：《中国中药杂志》2012年第6期832-835,共4页China Journal of Chinese Materia Medica

基　　金：湖北省教育厅项目(2009Z001)

摘　　要：目的:建立紫金砂药材的高效液相指纹图谱方法。方法:采用Kromasil 100A C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水为流动相梯度洗脱,流速为1 mL.min-1,检测波长为254 nm。以异欧前胡素为参照物,对10批紫金砂药材进行指纹图谱分析。结果:建立了紫金砂的HPLC指纹图谱共有模式,标定了8个共有峰,10批药材的相似度均>0.9。结论:该方法简便、准确、重复性好,为紫金砂药材的品质评价和质量控制标准的制定提供科学依据。Objective： To establish the HPLC fingerprint of Angelica polymorpha. Method： The 10 batches of A. polymorpha were measured by HPLC with isoimperatorin as a reference substance and the chromatographic experients were performed on Kromasil 100A C18 column （4. 6 mm × 250 mm, 5 um）, eluted with acetonitrile and water as mobile phase in gradient mode. The flow rate was 1.0 mL . min- 1 and the detection wavelength was 254 nm. Result： The common mode of the HPLC fingerprints were set up. There were 8 common peaks in the fingerprint of 10 samples, and the similarity of the 10 samples was more than 0. 9. Conclusion： The method is simple, accurate and have a good reaptability. The quality of A. polymorpha can be controlled effectively by the HPLC fingerprint.

关 键 词：紫金砂 HPLC 指纹图谱 

分 类 号：R284[医药卫生—中药学]