镉对凡纳滨对虾离体血细胞的毒性影响  

Toxic Effects of Cadmium on Haemocytes of Litopenaeus vannamei in Vitro

作　　者：冼健安[1] 王安利[1] 苗玉涛[1] 李彬[1] 潘训彬[1] 

机构地区：[1]华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,广东省高等学校生态与环境科学重点实验室,广州510631

出　　处：《水生态学杂志》2012年第1期112-115,共4页Journal of Hydroecology

基　　金：国家自然科学基金资助项目(30671628);国家科技支撑计划项目(2007BAD29B04;2007BAD29B06);广东省自然科学基金项目(S2011020003256);广东省重大科技专项(2009A060800019;2009A020101006);广东省教育部产学研结合资助项目(2006D90204005和2011B090500009);广州市科技支撑计划项目(2009Z1-E681)

摘　　要：对凡纳滨对虾(Litopenaeus vannamei)血细胞进行离体镉离子(Cd2+)应激试验。Cd2+浓度为10-3～10-9M,在应激6h后取样,利用流式细胞术(FCM)检测血细胞的活性和活性氧(ROS)含量。结果显示,当Cd2+浓度为10-5M时,血细胞的ROS含量显著升高;当Cd2+浓度为10-3和10-4M时,血细胞的死亡率和ROS含量均显著上升;表明Cd2+的细胞毒性作用与其浓度相关,Cd2+浓度达到10-5M时即可诱导血细胞ROS的产生,但当Cd2+浓度达到10-4M及以上时,被诱导产生的ROS对细胞造成氧化伤害,从而导致细胞死亡。Stress test of cadmium ion on haemocytes of Litopenaeus vannamei in vitro was conducted. Exposed to concentration of 10^-9 - 10^-3 M Cd^2＋ in 6 h in vitro, haemocyte viability and reactive oxygen species （ROS） of L. vannamei haemocyte samples were determined through flow cytometry （FCM）. The results showed that ROS con- tents in haemocytes surged significantly under the concentration of 10^-5 M cadmium; Under the concentration be- tween 10^-4 and 10^-3 M cadmium, mortality and ROS contents of haemocytes ascended emphatically respectively. These results showed the cytotoxicity of Cd^2＋ on L. vannamei was in relevance to the concentration of Cd^2＋. ROS of haemocytes would be induced with Cd^2＋ concentration reaching 10^-5 M, but the induced ROS would cause oxida- tive damage to haemocytes when Cd^2＋ concentration reaching 10^-4 M or above, thus contributing to haemocyte death.

关 键 词：镉 凡纳滨对虾 血细胞 流式细胞术 

分 类 号：Q355[生物学—遗传学]