In-line X-ray phase-contrast imaging of murine liver microvasculature ex vivo  

作　　者：LI Beilei XU Min WU Weizhong PENG Guanyun ZHANG Xi PENG Yifeng SHI Hongcheng CHEN Shaoliang 

机构地区：[1]Department of Nuclear Medicine,Zhongshan Hospital Affiliated to Fudan University,Shanghai 200032,China [2]Liver Cancer Institute,Zhongshan Hospital Affiliated to Fudan University,Shanghai 200032,China [3]Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China [4]Department of Radiology,Tumor Hospital Affiliated to Fudan University,Shanghai 200032,China [5]Department of Radiology,No.10 People's Hospital Affiliated to Tongji University,Shanghai 200072,China

出　　处：《Nuclear Science and Techniques》2012年第1期10-14,共5页核技术（英文）

基　　金：Supported by National Basic Research Program of China (973 Program Grant No.2010CB834305)

摘　　要：Imaging blood vessels is of importance for determining the vascular distribution of organs and tumors.Phase-contrast X-ray imaging can reveal the vessels in much more detail than conventional X-ray absorption method.Visualizing murine liver microvasculature ex vivo with phase-contrast X-ray imaging was performed at Shanghai Synchrotron Radiation Facility.Barium sulfate and physiological saline were used as contrast agents for the blood vessels.Blood vessels of <Φ20μm could be detected by replacing resident blood with physiological saline or barium sulfate.An entire branch of the portal vein (from the main axial portal vein to the ninth generation of branching) could be captured in a single phase-contrast image.It is demonstrated that selective angiography based on phase contrast X-ray imaging,with a physiological material of low Z elements (such as saline) being the contrast agent,is a viable imaging strategy.Further efforts will be focused on using the technique to image tumor angiogenesis.Imaging blood vessels is of importance for determining the vascular distribution of organs and tumors. Phase-contrast X-ray imaging can reveal the vessels in much more detail than conventional X-ray absorption method. Visualizing murine liver microvasculature ex vivo with phase-contrast X-ray imaging was performed at Shanghai Synchrotron Radiation Facility. Barium sulfate and physiological saline were used as contrast agents for the blood vessels. Blood vessels of 〈~520 paaa could be detected by replacing resident blood with physiological saline or barium sulfate. An entire branch of the portal vein （from the main axial portal vein to the ninth generation of branching） could be captured in a single phase-contrast image. It is demonstrated that selective angiography based on phase contrast X-ray imaging, with a physiological material of low Z elements （such as saline） being the contrast agent, is a viable imaging strategy. Further efforts will be focused on using the technique to image tumor angiogenesis.

关 键 词：X射线成像 相衬成像 微血管 体外 小鼠 上海同步辐射装置 肿瘤血管生成 生理盐水 

分 类 号：Q959.836[生物学—动物学] TP391.41[自动化与计算机技术—计算机应用技术]