热带石松科植物中石杉碱甲的含量差异(英文)  

Variations of huperzine A content in Lycopodiaceae species from tropics

作　　者：NorShahidah Sahidan Chee Yan Choo A.Latiff Razali Jaman 

机构地区：[1]MedChem Herbal Research Group Faculty of Pharmacy Universiti Teknologi MARA 42300 Selangor Malaysia [2]Faculty of Sciences and Technology Universiti Kebangsaan Malaysia Bangi Selangor Malaysia [3]Faculty of Sciences and Technology Universiti Kebangsaan Malaysia Bangi Selangor, Malaysia

出　　处：《中国天然药物》2012年第2期125-128,共4页

基　　金：supported by the eScience (No.UiTM 02-01-01-SF105) grant from Ministry of Science,Technology and Innovation

摘　　要：(-) Huperzine A is a bioactive alkaloid from Huperzia serrata (Lycopodiaceae) used for the treatment of Alzheimer's disease. High yielding (-) huperzine A species is of interest for mass propagation since it grows slowly in temperate countries. The content of (-) huperzine A from temperate countries was reported but none reported from tropical species. The aim of this study was to evaluate the content of (-) huperzine A from tropical club mosses and further identify a high yielding species. Club mosses from Lycopodiaceae family were collected from Peninsular Malaysia. The collected club mosses were dried, pulverized and extracted with methanol. A gradient reverse phase HPLC-photodiode array detector method with increasing amount of methanol in 0.01 % trifluoroacetic acid was developed. The calibration curve was linear from 5 to 100 g·mL-1 with correlation coefficient, r2, of 0.998 1. The precision for both intra-and inter-day peak area were between 0.48% to 1.24 % and 0.95% to 3.85 %, respectively. The recovery of the method was between 99.8% to 103.8 %. Though geographically segregated, (-) huperzine A content in Huperzia phlegmaria and H.carinata found in the tropics was highest and similar to species in Australasia. Both species may provide a good source of (-) huperzine A.（-） Huperzine A is a bioactive alkaloid from Huperzia serrata （Lycopodiaceae） used for the treatment of Alzheimer＇s disease. High yielding （-） huperzine A species is of interest for mass propagation since it grows slowly in temperate countries. The content of （-） huperzine A from temperate countries was reported but none reported from tropical species. The aim of this study was to evaluate the content of （-） huperzine A from tropical club mosses and further identify a high yielding species. Club mosses from Lycopodiaceae family were collected from Peninsular Malaysia. The collected club mosses were dried, pulverized and extracted with methanol. A gradient reverse phase HPLC-photodiode array detector method with increasing amount of methanol in 0.01 % trifluoroacetic acid was developed. The calibration curve was linear from 5 to 100 g·mL-1 with correlation coefficient, r2, of 0.998 1. The precision for both intra-and inter-day peak area were between 0.48% to 1.24 % and 0.95% to 3.85 %, respectively. The recovery of the method was between 99.8% to 103.8 %. Though geographically segregated, （-） huperzine A content in Huperzia phlegmaria and H.carinata found in the tropics was highest and similar to species in Australasia. Both species may provide a good source of （-） huperzine A.

关 键 词：LYCOPODIACEAE (-) Huperzine A HPLC TROPICS 

分 类 号：R917[医药卫生—药物分析学] R931[医药卫生—药学]