人面果叶子、根部、果实提取物体外抗氧化活性研究(英文)  

Antioxidant activity of Garcinia xanthochymus leaf,root and fruit extracts in vitro

作　　者：付蒙[1] 冯慧瑾 陈玉[3] 王德彬[1] 杨光忠[1] 

机构地区：[1]中南民族大学药学院,武汉430040 [2]中国科学院上海药物研究所新药研究国家重点实验室,上海201203 [3]中南民族大学化学与材料科学学院,武汉430040

出　　处：《中国天然药物》2012年第2期129-134,共6页

基　　金：supported by the State Key Laboratory of Drug Research (No.SIMM0901KF-02);the National Natural Science Foundation of China (No. 30670215)~~

摘　　要：目的:评价人面果叶子、根部、果实提取物体外抗氧化活性。方法:采用相应的实验体系测定总酚含量、还原能力、清除自由基能力、抑制DNA损伤作用,从而评价人面不同部位的石油醚提取物、乙酸乙酯提取物、正丁醇提取物、水提取物的抗氧化活性。结果:6个提取物显示抗氧化活性,其中叶子部位乙酸乙酯提取物的抗氧化活性与人工合成抗氧化剂BHT相当。结论:此次研究为从人面果中分离得到天然抗氧化剂提供理论基础。AIM： To investigate the antioxidant activity of Garcinia xanthochymus leaf, root and fruit extracts in vitro. METHODS： The antioxidant activity of petroleum ether, ethyl acetate, n-butanol and water fractions from each plant part of G. xanthochymus were assessed by determining the total phenolic content, evaluating the reducing power, measuring the capability for scavenging free radicals, as well as its ability to inhibit DNA cleavage, using appropriate assay systems. In addition, high performance liquid chromatography （HPLC） was used to quantify the Garcinia biflavone GB-1a in the fractions from each plant part of G. xanthochymus. RESULTS： Six fractions showed antioxidant activity, especially the ethyl acetate fraction from the leaf of G. xanthochymus showed a potent antioxidant activity which was similar to that of the standard antioxidant BHT. CONCLUSION： This study provided a theoretical reference for the further isolation of natural antioxidants from G. xanthochymu.

关 键 词：人面果 抗氧化 高效液相色谱 藤黄双黄酮 

分 类 号：R965[医药卫生—药理学]