丹参酮ⅡA在3T3-L1脂肪细胞中促进脂联素的组装(英文)  

Tanshinone ⅡA promotes the assembly of adiponectin in 3T3-L1 adipocytes

作　　者：林桓 李叶华 李赟 易骏阳 麦毓麟 李珍[1] 

机构地区：[1]清华大学生命科学学院,北京100084

出　　处：《中国天然药物》2012年第2期150-154,共5页

基　　金：supported by the National Natural Science Foundation of China (Nos. 30470354, 30570356)~~

摘　　要：目的:研究丹参酮IIA对3T3-L1脂肪细胞中脂联素表达和蛋白多聚化的作用。方法:用油红染色鉴定3T3-L1脂肪细胞的分化状态。用Real-time PCR检测基因的mRNA表达水平。用2%15%梯度SDS-PAGE及Western blot检测脂联素三种聚体。用siRNA技术进行基因沉默。结果:本研究发现传统中药丹参中的萘醌二萜类成分丹参酮IIA在脂肪细胞中激活AMPK通路,同时抑制脂肪细胞分化,降低脂联素的表达。而在前体脂肪细胞分化过程或成熟脂肪细胞中加入丹参酮IIA进行处理,均明显促进脂联素的多聚化,增加高聚体的浓度。抑制AMPK通路能够解除丹参酮IIA对脂联素的作用。结论:丹参酮IIA通过激活AMPK在3T3-L1脂肪细胞中促进脂联素的组装。AIM： To investigate the effect of tanshinone ⅡA on adiponectin biosynthesis. METHODS： The differentiation state of 3T3-L1 adipocytes was checked by Oil Red O staining. mRNA levels were examined by real-time PCR. Adiponectin oligomers were subjected to 2%-15% gradient SDS-PAGE and then detected using western blotting. Gene silencing experiments were performed using standard siRNA methods. RESULTS： It was found that tanshinone ⅡA, a diterpenoid naphthoquinone from the Chinese traditional herb Danshen （Salvia miltiorrhiza）, inhibited the differentiation of preadipocytes and down-regulated the expression of adiponectin. In contrast, tanshinone ⅡA promoted the assembly of high-molecular weight （HMW） adiponectin during differentiation and in mature adipocytes. Tanshinone ⅡA activated AMP-activated protein kinase （AMPK） in 3T3-L1 adipocytes, and suppression of the AMPK pathway blocked the effect of tanshinone ⅡA on adiponectin expression and multimerization. CONCLUSION： These data suggest that tanshinone ⅡA promotes the assembly of HMW adiponectin through a process involving AMPK activation.

关 键 词：脂联素 蛋白多聚化 丹参酮IIA AMPK通路 3T3-L1脂肪细胞 

分 类 号：R965[医药卫生—药理学]