电针对局灶性脑缺血模型大鼠皮层BDNF、TrkB的影响  

Influence of Electroacupuncture on the Expressions of BDNF and TrkB in Ischemic Cortex of Rats With Local Cerebral Ischemia

作　　者：王彦春[1] 梁少荣[1] 马骏[1] 甘水咏[1] 王述菊[1] 姜拯坤[1] 雷俊[1] 王培峻[1] 许永海[1] 陈剑明[2] 

机构地区：[1]湖北中医药大学,湖北武汉430061 [2]北京中医药大学,北京100029

出　　处：《中华中医药学刊》2012年第3期469-471,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30300461;30973787;30973809);湖北省教育厅科学技术研究资助项目(2002B00004;D200726001;Z20091601);湖北省科技厅科学技术资助项目(2009CDA068;2010CDA101);武汉市科学技术局科学技术研究资助项目(201060623269)

摘　　要：目的:观察电针对不同时间点局灶性脑缺血模型大鼠缺血区皮层BDNF、TrkB的影响。方法:采用线栓改良法复制大脑中动脉缺血模型(MCAO),采用免疫组化SABC法观察缺血皮层BDNF、TrkB表达情况。结果:BDNF、TrkB阳性神经元主要分布在梗死灶周围皮质区。假手术组大鼠相应区域可见少量阳性神经元表达,且强度较弱;模型组各时间点梗死周围皮质BDNF、TrkB阳性神经元比假手术组明显增多,差异有统计学意义(P<0.05);电针组各时间点梗死周围皮质BDNF、TrkB比相同时间点模型组显著增多,差异有统计学意义(P<0.05)。结论:电针风府穴可以增加急性脑缺血后缺血周围皮质BDNF、TrkB阳性神经元,对缺血性脑损伤起修复和保护作用。Objective：To observe the influence of electroacupuncture （EA）on the expressions of BDNF and its receptor TrkB in ischemic cortex of rats with local cerebral ischemia. Methods ： The model of focal cerebral ischemia was set up by blocking up the middle cerebral artery（MCA） with filament in rats. The expressions of BDNF and TrkB positive neurons in ischemic cortex was observed with the method of immunocytochemistry SABC. Results ： BDNF and TrkB positive neurons were mainly distributed in cortex around the ischemic focus. In pseudo -operation groups ,BDNF and TrkB positive neurons were few and low - expression in identical area. The expressions of BDNF and TrkB around ischemic cortex of model groups were significantly higher than that of pseudo - operation group （ P 〈 0.05 ） ; BDNF and TrkB positive neu- rons in EA group were more than that of model group（P 〈0.05）. Conclusion：EA at Fengfu has a protective effect on ischemic brain injury by increasing the BDNF and its receptor TrkB expressions in cortex around the ischemic focus.

关 键 词：脑缺血 电针 风府穴 脑源性神经营养因子(BDNF) 酪氨酸激酶B(TrkB) 

分 类 号：R285.5[医药卫生—中药学] R245.97[医药卫生—中医学]