正交试验设计优化桑叶总生物碱提取工艺  

Optimization of Extraction Technology of Total Alkaloids from Mulberry Leaves by Orthogonal Design

作　　者：邓伟杰[1] 孙智平[1] 罗新根[1] 吴新荣[1] 

机构地区：[1]广州军区广州总医院药剂科,广东广州510010

出　　处：《中华中医药学刊》2012年第3期608-609,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：广东省科技计划资助项目(2008A030201031)

摘　　要：目的:利用正交试验设计研究桑叶总生物碱的最佳提取工艺。方法:以煎煮时间和加水量为考察因素,以总生物碱含量为评价指标,采用正交试验优选最佳水煎煮提取工艺条件。结果:优选的最佳提取工艺为用12倍量水煎煮1h。结论:优选得到的工艺稳定、可行,提取得到桑叶总生物碱含量高。Objective ： To study the best extraction technology of total alkaloids from mulberry leaves by orthogonal design. Method ：The extraction technology of total alkaloids from mulberry leaves was optimized by orthogonal experiment with the extraction time and the amount of solvent as factors and with the content of total alkaloids as index. Result：The optimum extraction condition was that the materials were extracted for 1 hour with 12 times amout of water. Conclusion： The technology after optimization had a good stability, the yield of total alkaloids was high, and the method was easy to be operated.

关 键 词：桑叶 总生物碱 提取工艺 正交试验 

分 类 号：R284.1[医药卫生—中药学]