基于碳纳米管/L-半胱氨酸/Fe_3O_4@Au纳米复合材料的电流型甲胎蛋白免疫传感器的研究  

An Immunosensor for α-Fotoprotein Antigen Based on Multi-wakked Nanotubes/L-Cysteine and Au@ Fe_3O_4 Nanocomposite

作　　者：朱宇萍[1,2] 袁若[2] 柴雅琴[2] 覃松[1] 袁亚利[2] 

机构地区：[1]内江师范学院化学化工学院,内江641112 [2]西南大学化学化工学院,重庆400715

出　　处：《分析化学》2012年第3期359-364,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20675064);重庆市自然科学基金(No.CSTC-2005 BB 4100)资助项目

摘　　要：将DMF(N,N-二甲基甲酰胺)分散的多壁碳纳米管(MWNT)修饰在金电极表面,再将修饰电极依次沉积纳米金和L-半胱氨酸(L-Cys),并通过半胱氨酸中的巯基吸附Fe3O4@Au纳米复合材料,再固载甲胎蛋白抗体(anti-AFP),以牛血清白蛋白(BSA)封闭非特异性吸附位点,构建了高灵敏、稳定的新型电流型甲胎蛋白免疫传感器。实验通过扫描透射电子显微镜(TEM)对DMF-MWNT和Fe3O4@Au复合纳米粒子进行了表征。在优化的实验条件下,此免疫传感器对甲胎蛋白抗原的检测范围为0.1～150μg/L,检出限为0.03μg/L。N,N-Dimethylfomamide（DMF） dispersed MWNTS was firstly modified on the electrode surface,which was in further eletrodeposited a nano-Au layer for immobilization of L-cysteine.Subsequently,Fe3O4@Au nanocomposites were absorbed on the modified electrode surface by the SH-Au bond for increasing the specific surface area and further immobilizing anti-AFP.Finally,bovine serum albumin（BSA） was used to block the non-specific adsorption sites of the immunosensor to obtain a highly sensitive and stable immunosensor.Experiments by transmission electron micros-copy（TEM） were carried out to characterize the prepared MWCNTs and Fe3O4@Au composite nanoparticles.Under optimal conditions,the sensor has a good response to AFP,in the detection range form 0.1 to 150 μg/L,and detection limit of 0.03 ng/mL.

关 键 词：Fe3O4@Au纳米复合粒子 碳纳米管 L-半胱氨酸 甲胎蛋白 免疫传感器 

分 类 号：TB383.1[一般工业技术—材料科学与工程]