正常状态下黑线仓鼠脑内c-fos的基础表达  

The Basal Expression of C-fos in The Brain of Cricetulus Barabensis Under Normal Conditions

作　　者：李广宇[1] 宋倩[1] 李亚[1] 徐来祥[1] 

机构地区：[1]曲阜师范大学生命科学学院,山东曲阜273165

出　　处：《科技信息》2012年第5期50-51,共2页Science & Technology Information

基　　金：国家自然科学基金项目(31070332);山东省自然科学基金项目(ZR2009DL009)

摘　　要：目的:研究正常状态下黑线仓鼠脑内前脑皮层、海马和下丘脑区c-fos的基础表达。方法:采用免疫组织化学方法(SABC)检测c-fos蛋白在黑线仓前脑皮层、海马和下丘脑区的表达,以昆明品系小鼠为对照,建立足底电击急性应激小鼠模型。结果:正常生活状态下黑线仓鼠脑内前脑皮层、海马和下丘脑区c-fos蛋白广泛表达;其中海马CA1和CA3区c-fos阳性神经元相对数量较少,海马DG区、前脑皮层和下丘脑区c-fos阳性神经元相对数量较多;与正常小鼠相比,前脑皮层c-fos阳性神经元显著增多(P<0.01)。应激小鼠各脑区c-fos阳性神经元数量明显增多,与正常小鼠比较有统计学显著差异(P<0.05,P<0.01)。各脑区c-fos阳性神经元平均目标灰度值结果类似。结论:正常状态下黑线仓鼠脑内c-fos蛋白的表达可能与动物的行为活动和内分泌、空间认知功能以及复杂运动的调控密切相关。Objective： To study the basal expression of c-fos in the Prefrontal cortex（PFC）,Hippocampus（HP） and Hypothalamus（HY） of Cricetulus barabensis under normal conditions.Methods： The expression of c-fos proteins in the HP,PFC and HY were detected by immunohistochemical method.Kunming mice were used as the control.The acute stress model mice with single-factor stressors（foot shock） were applied.Results： There was a wide expression of c-fos proteins in the PFC,HP and HY of Cricetulus barabensis under normal conditions.The number of c-fos positive neurons in CA1 and CA3 area of HP were a small amount,and that were more in dentate gyrus（DG） of HP,PFC and HY.Compared with normal mice,the number of c-fos positive neurons in the PFC were significantly increased in Cricetulus barabensis（P0.01）.The number of c-fos positive neurons in the PFC,HP and HY were significantly increased in stress mice（P0.05,P0.01）,compared with normal mice.And that the changes of the gray scale value of c-fos positive neurons in PFC,HP and HY were similar too.Conclusion： The expression of c-fos proteins in Cricetulus barabensis under normal conditions may be nearly related to the animal behaviour and endocrine,spatial cognitive function and modulating control of voluntary movement.

关 键 词：黑线仓鼠 C-FOS 前脑皮层 海马 下丘脑 

分 类 号：Q42[生物学—神经生物学]