微波处理对嗜碱和嗜盐海洋放线菌分离效果的影响  

Effects of microwave irradiation on isolation of basophilic and halophilic marine actinomycetes

作　　者：丁彦博[1] 蔡超靖[1] 穆云龙[1] 单越琦[1] 路新华[1] 蒋沁[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司微生物药物国家工程研究中心河北省工业微生物代谢工程技术研究中心,河北石家庄050015

出　　处：《微生物学通报》2012年第3期407-414,共8页Microbiology China

基　　金：国家自然科学基金项目(No.30873107);国家973计划项目子课题(No.2009CB526513)

摘　　要：【目的】研究微波处理对于分离嗜碱和嗜盐海洋放线菌的效果。【方法】用微波处理7份海泥样品,梯度稀释后涂布于3种分离培养基,分离具有嗜碱和嗜盐特性的海洋放线菌。【结果】微波处理后的7份样品中,4份样品中嗜碱海洋稀有放线菌和3份样品的嗜盐海洋稀有放线菌数量极显著提高;7份样品中的嗜碱、嗜盐海洋小单孢菌属、游动放线菌属、诺卡氏菌属等稀有放线菌数量均有显著增加,不同样品中新分离到链孢菌属、小双孢菌属、链孢囊菌属及其他未鉴定的海洋稀有放线菌,分离到属的数量提高了1-4个。【结论】微波处理不仅显著提高嗜碱和嗜盐海洋放线菌的分离数量,而且明显增加了海洋稀有放线菌的分离种类。[Objective] Study the effects of microwave irradiation on isolation of basophilic and halophilic marine actinomycetes. [Methods] Seven marinemud samples were radiated by mi- crowave and then gradient diluted for isolation of basophilic and halophilic marine actinomycetes in three media. [Results] Microwave irradiation could highly significantly increase the to- tal quantity of basophilic and halophilic marine actinomycetes respectively in four and three marinemud samples. The total quantity of basophilic and halophilic marine rare actinomycetes of Micromonospora, Actinoplanes and Nocardia were significantly increased after microwave irradiation. The species of other marine rare actinomycetes such as Catellatospora, Microbispora, Streptosporangium were increased to one to four in different samples. [Conclusion] Microwave irradiation could significantly increase the total quantity of basophilic and halophilic marine actinomycetes and the species of marine rare actinomycetes.

关 键 词：微波 嗜碱 嗜盐 海洋放线菌 分离 

分 类 号：Q93-3[生物学—微生物学]