肺炎支原体感染在BALB/c小鼠肺组织不同时间炎症的变化  

Changes of lungs in BALB/c mouse after mycoplasma pneumonia infection in different periods

作　　者：张晗[1] 尚云晓[1] 周潇男[2] 何梦博[3] 焦旭勇[1] 王莉[1] 韩晓华[1] 王桂珍[3] 

机构地区：[1]中国医科大学附属盛京医院小儿呼吸内科,辽宁沈阳110004 [2]中国医科大学,94期学生辽宁沈阳110005 [3]中国医科大学基础医学院病生原物学教研室,沈阳110005

出　　处：《解剖学研究》2012年第1期8-11,共4页Anatomy Research

基　　金：辽宁省教育厅课题(L2011128)

摘　　要：目的了解肺炎支原体感染的不同时期肺组织的病理变化、用PCR的方法检测肺炎支原体出现时间、阳性率的变化情况。方法建立肺炎支原体经呼吸道感染的BALB/c小鼠模型,对感染后3、7、14、21 d的小鼠的肺组织进行病理变化、肺湿重、PCR检测肺炎支原体出现时间的研究。结果肺炎支原体经呼吸道感染的BALB/c小鼠肺组织变大重量增加,肺指数同正常对照组相比,P﹤0.01,差异有统计学意义;呼吸道感染的BALB/c小鼠肺组织在3、7 d时,肺组织的病理变化最明显,14 d炎症减轻,21 d炎症基本消退;用PCR的方法检测支气管肺泡灌洗液肺炎支原体7 d时全部为阳性。结论肺炎肺炎支原体感染BALB/c小鼠模型制作成功,感染后7 d时肺部炎症最明显。Objective To study the changes of pathology and positive expression of mycoplasma pneumonia（MP）in lungs of BALB/c mice by PCR after mycoplasma pneumonia infection.Methods The mycoplasma pneumonia infection model was builded by inhalation in BALB/c mouse;the pathology and the positive expression of MP were detected by PCR on 3 days,7 days,14 days,21 days after mycoplasma pneumonia infection.Results Compare with control group,the lungs of BALB/c mice infected by mycoplasma pneumonia were larger and heavier（P﹤0.01）;the inflammation in lungs tissue of mice were significantly on 3 days and 7 days,and alleviated on 14 days,disappeared on 21 days;the MP positive expression was observed in bronchoalveolar lavage fluid（BALF） on 7days after mycoplasma pneumonia infection.Conclusion BALB/c mice is succsessfully infecte by mycoplasma pneumonia,and inflammation is significantly on 7 day after MP infection.

关 键 词：肺炎支原体 BALB/C小鼠 动物模型 

分 类 号：R725.6[医药卫生—儿科]