大鼠垂体促性腺激素细胞内cAMP的改变对LHβ mRNA表达的影响  

Effect of the changes of cAMP in pituitary gonadotropic hormone(GTH)cells on the expression of LH mRNA in rats

作　　者：王新[1,2] 赖小平[2] 谭建华[3] 韦旭斌[4] 

机构地区：[1]青岛农业大学动物科学技术学院,青岛266109 [2]广州中医药大学中药学院,广州510006 [3]解放军军事医学科学院军事兽医研究所,长春130062 [4]吉林大学畜牧兽医学院,长春130062

出　　处：《中国实验动物学报》2012年第1期10-13,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(No.30571356);广东省科技计划项目(NO.2011B050300021);青岛农业大学课题(No.630810;No.SYJK09-05;No.XJG0906);广州中医药大学博士后科研启动金(No.B3YH1008)资助项目

摘　　要：目的分析大鼠LHβ mRNA表达的促性腺激素释放激素(GnRH)受体后信号转导机制。方法将体外培养的大鼠腺垂体促性腺激素(GTH)细胞用cAMP的兴奋剂FSK或抑制剂SQ22536处理后,再用高频GnRH脉冲刺激,然后用实时荧光定量PCR法测定细胞LHβ mRNA的Ct值,并与空白组比较。结果 LHβ mRNA的Ct值随着GTH细胞cAMP含量的增高而显著降低,随着cAMP含量的降低而显著增高。结论 cAMP是高频GnRH脉冲刺激所引起的LHβ mRNA表达的受体后的信号转导途径。Objective Try to analyze the gonadotropin-releasing hormone （GnRH） post-receptor signal transduction mechanism of the expression of LHI3 mRNA in Rats. Methods The gonadotropic hormone （GTH） cells of rat adenohypophysis was stimulated by GnRH impulse at high frequency after cAMP in them was excited by forskolin （FSK） or inhibited by SQ 22536. The Ct value of LHβ mRNA was detected by real-time quantitative polymerase chain reaction, and was compared with that of the blank control group. Results The Ct value of LHβ mRNA was significantly falling with the rise of cAMP in GTH ceils and significantly rising with the falling of cAMP. Conclusions The cells stimulated by GnRH impulse at high frequency respond to LH βmRNA and cAMP is the post-receptor signal transduction pathway.

关 键 词：促性腺激素释放激素 促性腺激素 信号转导 大鼠 

分 类 号：Q95-33[生物学—动物学]