Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis  

作　　者：Serafin Perez-Cerezales： Alberto Miranda Alfonso Gutierrez-Adan 

机构地区：[1]Department of Animal Reproduction, National Institute for Agricultural and Food Research and Technology, Madrid 28040, Spain

出　　处：《Asian Journal of Andrology》2012年第2期335-337,共3页亚洲男性学杂志（英文版）

摘　　要：It is well known that transit through the epididymis involves an increase in the compaction of sperm chromatin, which acquires fully condensed status at the caput epididymidis. The purpose of this study was to compare the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling （TUNEL） assay, the comet assay, the sperm chromatin structure assay （SCSA） and the sperm chromatin dispersion （SCD） test by analysing spermatozoa from the caput and cauda epididymidis in order to demonstrate the ability of each technique to discriminate between different degrees of sperm maturity related to chromatin compaction and DNA fragmentation. Our results suggest that some populations of DNA-fragmented spermatozoa associated with immature sperm can only be identified using the comet assay and the SCSA but not with the SCD test or the TUNEL assay.It is well known that transit through the epididymis involves an increase in the compaction of sperm chromatin, which acquires fully condensed status at the caput epididymidis. The purpose of this study was to compare the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling （TUNEL） assay, the comet assay, the sperm chromatin structure assay （SCSA） and the sperm chromatin dispersion （SCD） test by analysing spermatozoa from the caput and cauda epididymidis in order to demonstrate the ability of each technique to discriminate between different degrees of sperm maturity related to chromatin compaction and DNA fragmentation. Our results suggest that some populations of DNA-fragmented spermatozoa associated with immature sperm can only be identified using the comet assay and the SCSA but not with the SCD test or the TUNEL assay.

关 键 词：DNA damage mouse epididymis sperm chromatin sperm maturation the comet assay the sperm chromatin dispersion test the sperm chromatin structure assay TUNEL 

分 类 号：Q523.03[生物学—生物化学] S823[农业科学—畜牧学]