百会穴注射腺苷A1受体激动剂对脑缺血再灌注损伤大鼠大脑皮质的影响  

Effects of Injecting Adenosine A1 Receptor Agonist into Baihui(GV20) on the Cerebral Cortex in Ischemia/Reperfusion Injury Model Rats

作　　者：戴勤学[1] 王雷雷[1] 晁娟[1] 王宏法[1] 刘俊伟[1] 罗亮[1] 王均炉[1] 陈必成[2] 

机构地区：[1]温州医学院附属第一医院麻醉科,浙江温州325000 [2]温州医学院附属第一医院外科实验室,浙江温州325000

出　　处：《中国中西医结合杂志》2012年第3期390-393,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省自然科学基金资助项目(No.Y2101225);浙江省中医药管理局(重点项目)(No.2009ZA013)

摘　　要：目的观察百会穴(GV20)注射腺苷A1受体激动剂(2-chloro-N6-cyclopentyladenosine,CCPA)对局灶性脑缺血再灌注损伤大鼠大脑皮质的影响。方法将24只SD大鼠随机分成假手术组、模型组、二甲基亚砜(DMSO)组和CCPA组。采用线栓暂时性阻塞大脑中动脉法制备局灶性脑缺血再灌注损伤模型;DMSO组和CCPA组分别在缺血再灌注即刻百会穴注射DMSO20μL和CCPA(0.1mmol/L)20μL;分别对大鼠行为学、大脑皮质半暗带区组织形态、Bcl-2蛋白表达量和神经细胞凋亡率进行评估。结果与模型组和DM-SO组比较,CCPA组大鼠行为学明显改善(P<0.05);神经元无明显的胞浆空染、核固缩等现像;Bcl-2蛋白表达量明显升高(P<0.01);细胞凋亡数明显减少(P<0.01)。结论百会穴注射CCPA能够改善脑缺血再灌注损伤大鼠行为学和大脑皮质半暗带区组织形态学,提高大脑皮质半暗带区Bcl-2蛋白表达量并减少神经细胞凋亡率,缓解大鼠脑缺血再灌注损伤,可作为一种新型的治疗方法。Objective To investigate the effects of injecting adenosine A1 receptor agonist（CCPA） into Baihui（GV20） on the cerebral cortex induced by the ischemia/reperfusion of middle cerebral artery occlusion（MCAO） in rats.Methods Twenty-four SD rats were randomly divided into four groups,i.e.,the sham-operation group,the model group,the DMSO group,and the CCPA group.The MCAO model was established by thread embolism method.At the moment of ischemia/reperfusion,the rats in DMSO group and the CCPA group were injected with DMSO（20 μL） and CCPA（0.1 mmol） 20 μL into Baihui respectively.The rats＇ behavior,the histomorphology of ischemic penumbra in the cerebral cortex,the expressions of Bcl-2 protein,and the apoptosis rate of neurocytes were assessed.Results Compared with the model group and the DMSO group,the rats＇ behavior were markedly improved in the CCPA group（P0.05） .No obvious karyopyknosis and cytoplasm empty dye of neurons appeared.The Bcl-2 expressions in rats＇ cerebral cortex obviously increased（P0.01） .The apoptosis number of neurons obviously decreased（P0.01） .Conclusions Injecting CCPA into Bahui improved the rats＇ behavior and histomorphology in the ischemic penumbra,elevated the expressions of Bcl-2 protein,and reduced the neurons apoptosis rate in the ischemic penumbra.It alleviated the cerebral ischemia-reperfusion injury.Therefore,it could be taken as a new treatment method.

关 键 词：穴位注射 腺苷A1受体激动剂 脑缺血再灌注 脑保护 

分 类 号：R245[医药卫生—针灸推拿学]