乙醛脱氢酶2在糖尿病大鼠心肌缺血/再灌注损伤中的抗凋亡作用  

Anti-apoptotic role of mitochondrial aldehyde dehydrogenase 2 in myocardial ischemia/reperfusion injury in diabetic rats

作　　者：王洪巨[1] 康品方[1] 叶红伟[2] 于影[2] 王晓梅[2] 高琴[2,3] 

机构地区：[1]蚌埠医学院第一附属医院心内科,安徽蚌埠233004 [2]蚌埠医学院生理学教研室 [3]安徽省组织移植重点实验室,安徽蚌埠233030

出　　处：《南方医科大学学报》2012年第3期345-348,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81000074);安徽省自然科学基金(090413097)~~

摘　　要：目的观察乙醛脱氢酶2(ALDH2)在糖尿病大鼠心肌缺血/再灌注凋亡发生中的作用。方法大鼠分为正常组、糖尿病组和ALDH2激动剂乙醇+糖尿病组。4周后行离体心肌缺血/再灌注(I/R)。测定复灌期间冠脉流出液中乳酸脱氢酶(LDH)含量。检测心肌组织细胞ALDH2、caspase-3的活性;RT-PCR测定左心室前壁心尖组织Bcl-2、Bax mRNA的表达。结果与正常大鼠I/R相比,糖尿病大鼠复灌期冠脉流出液中LDH释放增加,心肌组织caspase-3活性增加,ALDH2活性降低,Bcl-2/Bax mRNA比值降低;与糖尿病大鼠心肌I/R相比,ALDH2激动剂乙醇使得心肌复灌期间冠脉流出液中LDH释放减少,心肌caspase-3活性降低,ALDH2活性增高,Bcl-2/Bax mRNA比值增高。结论增强ALDH2在糖尿病大鼠心肌中的表达对缺血/再灌注损伤有明显的保护作用;其机制可能与抑制细胞凋亡的发生有关。Objective To evaluate the anti-apoptotic effect of aldehyde dehydrogenase 2（ALDH2） on myocardial ischemia/reperfusion（I/R） injury in diabetic rats.Methods Normal male SD rats were divided into normal,diabetes and ethanol（the agonist of ALDH2） ＋ diabetes groups.In the latter two groups,diabetes was induced by an intraperitoneal injection of 55 mg/kg STZ.Four weeks after the modeling,myocardial I/R was mimicked ex vivo,and lactate dehydrogenase（LDH） content in the coronary flow was determined.The activities of caspase-3 and ALDH2 were evaluated,and the expressions of Bcl-2 and Bax mRNA in the left anterior myocardium were detected using RT-PCR.Results In diabetic group,LDH release and caspase-3 activity were increased,while ALDH2 activity and Bcl-2/Bax mRNA expression were decreased as compared to those in normal control group.Compared with the diabetic group,ALDH2 agonist ethanol significantly reduced LDH release and caspase-3 activity,increased ALDH2 activity and Bcl-2/Bax mRNA expression.Conclusion In diabetic rats,enhanced ALDH2 expression can offer mycardial protection possibly in relation to suppress cell apoptosis.

关 键 词：乙醛脱氢酶2 心脏 糖尿病 缺血/再灌注损伤 凋亡 

分 类 号：R587.1[医药卫生—内分泌]