牛蒡子苷对晚期氧化蛋白产物诱导小鼠足细胞转分化的影响  

Effect of arctiin on mouse podocyte epithelial-mesenchymal transition induced by advanced oxidation protein products

作　　者：章俊[1] 郭婷婷[1] 杨蕾[1] 杜庆生[1] 华洁[1] 刘蓉芝[1] 汤珣[1] 

机构地区：[1]南方医科大学珠江医院肾内科,广东广州510282

出　　处：《南方医科大学学报》2012年第3期379-382,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(S2011010004053);广州市科技计划重点项目(11C22120703)

摘　　要：目的探讨牛蒡子苷对晚期氧化蛋白产物(AOPP)诱导小鼠足细胞转分化的影响。方法用200μg/ml AOPP刺激小鼠足细胞24 h,同时加入牛蒡子苷(浓度分别为50、100、200、400μmol/L)进行干预,采用Western blotting检测内质网应激标志性蛋白Grp78、CHOP及平滑肌激动蛋白(α-SMA)表达水平。结果牛蒡子苷干预后可抑制小鼠足细胞Grp78、CHOP、α-SMA蛋白表达水平,且在一定范围内呈剂量依赖性。结论 AOPP可通过引起小鼠足细胞内质网应激(ERS)从而导致上皮-间充质转分化(EMT),而牛蒡子苷则可通过减轻ERS从而逆转EMT,为治疗肾脏纤维化提供新的研究方向。Objective To study the effect of arctiin on mouse podocyte epithelial-mesenchymal transition（EMT） induced by advanced oxidation protein products（AOPP）.Methods Mouse podocytes were stimulated by 200 μg/ml AOPP for 24 h in the presence of 50,100,200,and 400 μmol/L arctiin.The expressions of α-smooth muscle actin,Grp78 and CHOP were detected using Western blotting.Result The expressions of α-SMA,Grp78 and CHOP were inhibited by arctiin,showing a dose-dependent effect within a given range of arctiin concentration.Conclusion AOPP causes endoplasmic reticulum stress to induce EMT of mouse podocytes,and arctiin can decrease EMT by alleviating the stress.This finding sheds light on a new scope of research of renal fibrosis.

关 键 词：牛蒡子苷 足细胞 上皮-间充质转分化 晚期氧化蛋白产物 

分 类 号：R285.5[医药卫生—中药学]